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Strain
B6;129S1-Map2k3tm1Flv +/-/J

Summary Data Summary
Official Name B6;129S1-Map2k3tm1Flv +/-/J
Common Name B6;129S1-Map2k3tm1Flv +/-/J
Description A targeting vector containing neomycin resistance and herpes
simplex virus thymidine kinase genes was used to disrupt
exons 8 and 9. The construct was electroporated into
129S1/Sv-p+ Tyr+ Kitl+ derived W9.5 embryonic stem (ES)
cells, and correctly targeted ES cells were injected into
C57BL/6 blastocysts. The resulting chimeric animals were
crossed to C57BL/6N mice for at least 10 generations. Upon
arriving at The Jackson Laboratory, the mice were crossed
with C57BL/6J at least once to establish the colony.SNP
(single nucleotide polymorphism) analysis performed by The
Jackson Laboratory revealed that this strain is a mixed
genetic background (5 out of 27 markers were segregating for
non-C57BL/6 alleles).
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
Phenotype Description Mice that are homozygous for the targeted mutation are
viable, fertile, normal in size and do not display any gross
physical or behavioral abnormalities. However, the donating
investigator reports that reproductive performance is poor
for unknown reasons. No gene product (mRNA or protein) is
detected by Northern blot analysis of liver and kidney or
Western blot analysis of peritoneal macrophages. Mutant mice
exhibit an impaired type I cytokine immune response with
reduced induced interferon gamma production. Macrophages
from mutant mice have a reduced p39 MAPK activation and
IL-12 production when challenged with LPS
lipopolysaccharide). Dendritic cells also have reduced IL-12
production response. Mutant mouse embryo fibroblasts (MEF)
have a reduced cytokine expression response to tumor
necrosis factor (TNF). This mutant mouse strain may be
useful in studies of inflammatory response.
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1


Investigators
NameInstitution
Praveen MannamYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Map2k3knockoutwildNot Specified


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