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Strain
B6.129S4-Ndufs4tm1Rpa/J

Summary Data Summary
Official Name B6.129S4-Ndufs4tm1Rpa/J
Common Name B6.129S4-Ndufs4tm1Rpa/J
Description An frt-flanked neo cassette with a 5' loxP site was inserted
downstream of exon 2 and an additional loxP site was
inserted upstream of exon 2. Germ line, flp mediated
recombination was used to remove the neo cassette. Exon 2
contains the mitochondrial targeting sequence.
Exon 2, encoding the last part of a mitochondrial targeting
sequence and the first 17 amino acids of the mature protein,
was flanked by loxP sites and an FRT-flanked SV-Neo cassette
was introduced to intron 5 using 129S4/SvJaeSor-derived
AK18.1 embryonic stem (ES) cells. Resultant mice were
crossed with Gt(ROSA)26Sor-FLPo mice (see Stock No. 007844)
to excise the neo cassette. The line was backcrossed to
C57BL/6J for more than 20 generations by the donating lab.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 1


Investigators
NameInstitution
John YoonUniversity of California Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Ndufs4knockoutknockoutNot Specified


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