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Strain
C57BL/6J-Tg(Pomc-EGFP)1Low/J

Summary Data Summary
Official Name C57BL/6J-Tg(Pomc-EGFP)1Low/J
Common Name C57BL/6J-Tg(Pomc-EGFP)1Low/J
Description Mice hemizygous for this POMC-EGFP transgene are viable and
fertile, with EGFP expression directed to POMC-expressing
neurons by the mouse Pomc (pro-opiomelanocortin-alpha)
promoter/enhancer regions. The donating investigator reports
that transcripts from the transgene encode EGFP but do not
express any POMC prohormone or peptides. Direct EGFP
fluorescence is observed in the arcuate nucleus of the
hypothalamus (ARC), in melanotrophs/corticotrophs of the
pituitary gland, and (unlike other POMC promoter-driven
fluorescent mice) in a subpopulation of newly born granule
neurons of the dentate gyrus of the hippocampus. EGFP
expression is also observed in the nucleus of the solitary
tract of the medulla. EGFP expression is downregulated as
neurons mature and migrate deeper into the granule cell
layer. These POMC-EGFP mice may be useful in studying
neuronal signaling pathways, energy metabolism, leptin
activity, obesity, seizures, depression, and epilepsy.
A 23 kb mouse Pomc cosmid clone (P27*3delta; containing 13
kb of 5' flanking sequences and 2 kb of 3' flanking
sequences) was modified by inserting an enhanced green
fluorescent protein (EGFP) cassette with polyA signal into
exon 2. This inserted the EGFP at a position between the
splice acceptor site and the translational start site of
Pomc locus on the cosmid. This entire POMC-EGFP transgene
was microinjected into pronuclei of one-cell-stage embryos
of C57BL/6J mice. One founder was generated and bred to
wildtype C57BL/6J mice to generate the transgenic colony.
The donating investigator reports that POMC-EGFP mice were
subsequently bred to C57BL/6J wildtype mice for at least 12
generations prior to arrival at The Jackson Laboratory. The
donating investigator also reports approximately 10 copies
of the transgene at a single insertion site. Upon arrival,
mice were bred with C57BL/6J inbred mice (Stock No. 000664)
for at least one generation to establish the colony.
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Experiments 2


Investigators
NameInstitution
Zoe ThompsonUniversity of Michigan-Ann Arbor


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