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Strain

B6.129P2-Acsl1^tm1Rcol/J

Summary

Data Summary

Official Name	B6.129P2-Acsl1^tm1Rcol/J
Common Name	B6.129P2-Acsl1^tm1Rcol
Description	A targeting vector containing FRT site flanked MC1-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 2. This construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells which were transiently transfected with a FLP1 (flpE) recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 7 generations.
Development Status	Phenotyping ongoing
Creation Method	knockin
Breeding Type	intercross
Phenotype Description	The isozyme encoded by the acyl-CoA synthetase long-chain family member 1 (Acsl1) gene catalyzes the synthesis of acyl-CoA from fatty acids that are between 10 and 22 carbons in size. These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. When bred to a strain with Cre recombinase expression in the liver (see Stock No. 003574 for example), the resulting mutant mouse strain exhibits a 50% decrease in total hepatic acyl-CoA synthetase enzyme activity and may be useful in studies of lipid metabolism and liver physiology.

Type	Count
Investigators	1
Genomics - Modifications	1
Experiments	3

Investigators

Name	Institution
Rosalind Coleman	University of North Carolina at Chapel Hill

Genomic Information

Gene	Allele 1	Allele 2	Protocol
Acsl1	knockin (lox)	knockin (lox)	Not Specified

OrderID	Order	Investigator	Experiment	Status	Measurements
21921	21921	Rosalind Coleman	ACSL KO clamp	Completed	264
21921	21921	Rosalind Coleman	ASCL KO CLAMS	Completed	240
1073	1073	Rosalind Coleman	GPAT KO Liver and adipose LCCoA	In Progress	406

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