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Measurement of energy expenditure, RER and body composition in 12 month old mice
placed on one of 3 diets(control (AIN93G), ketogenic (90% fat, 10% protein, 0.7%
carbohydrate), or low carb (70%fat, 20% protein, 10% carbohydrate)) for 1 month.
Background/Purpose: To measure energy expenditure and body composition in 12
month old mice placed on one of 3 diets(control (AIN93G), ketogenic (90% fat,
10% protein, 0.7% carbohydrate), or low carb (70%fat, 20% protein, 10%
carbohydrate)) for 1 month prior to analysis. All animals are fed 11.2 kcal/day
of their designated diet. This is one timepoint (12 mo) of a cross-sectional
study and a component of a larger longitudinal study looking at the effects of
the different diets on lifespan and aging.
Applicable research area(s): Obesity, Metabolism
day(s) post-natal (d)
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Curation Info (# flags)
Name / Abbreviation
Experimental Factor: Experimental Group
This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor: Mouse Diet
based on AIN93G; caloric density=3.95 kcal/g
Ketogenic diet (90% fat, 10% protein, 0.7% carbohydrate); caloric density= 6.53 kcal/g
low carbohydrate diet (70%fat, 20% protein, 10% carbohydrate); caloric density=5.61 kcal/g
Experimental Factor (Units)
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energy intake (24 hr)
energy intake (24 hr)
oxygen consumption (Light Period)
fat body mass
fat body mass
lean body mass
lean body mass
respiratory exchange ratio (Light Period)
bone mineral density
bone mineral content
fat tissue, %
% body fat
heat (Light Period)
oxygen consumption (Dark Period)
carbon dioxide production Light Period
carbon dioxide production Dark Period
respiratory exchange ratio (Dark Period)
heat (Dark Period)
carbon dioxide production
respiratory exchange ratio
energy intake (Light Period)
food intake - light
food intake (Dark Period)
food intake - dark
energy intake (avg 24 hr)
energy intake avg 24
energy intake (Ligth Period)
energy intake -light
energy intake (Dark Period)
energy intake - dark
Activity 24h (total x-axis)
Activity 24h total x
Activity 24h (x-ambulatory)
Activity 24h (x-amb)
Activity 24h (total vertical z-axis)
Activity 24h total z
total tissue mass
heat - 24h
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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