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Experiment

Metabolic Clamp Study in Osteocyte-GLUT4 KO Mice
Summary Data Summary
Investigator Clemens, Thomas
Description We will characterize the mechanisms through which insulin regulates glucose
transport in the osteoblast and determine the role of the osteoblast glucose
transporter4 in global glucose utilization. Finally, to test the validity of
putative bone pancreas endocrine loop in vivo, we will determine the effect of a
hyperinsulinemic euglycemic clamp on circulating undercarboxylated OC levels in
both wild type and mice lacking Glut4 in osteoblasts. In essence, these studies
will test the novel hypothesis that the osteoblast plays a critical role in the
maintenance of glucose homeostasis.

Disruption of Glut4 transporter in osteoblasts in vitro eliminated
insulin-stimulated glucose uptake, reduced proliferation and diminished measures
of osteoblast maturation. Glut4 Ob-KO mice exhibited increased peripheral
adiposity in association with mild hyper-insulinemia but marked insulin
resistance measured by ITT.

Applicable research area(s): Metabolism
Status Completed
Public Release 12/10/2017
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals14
Experimental Conditions1
Catalog Items3
Curation Info (# flags)2
Phenotype Assays13
Phenotype Measurements182
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
6
0
Glut4lox/lox Tg(BGLAP-cre)1Clem
0
8
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.



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