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Experiment

Metabolic Clamp Study in Osteocyte-GLUT4 KO Mice

Summary

Data Summary

Investigator	Clemens, Thomas
Description	We will characterize the mechanisms through which insulin regulates glucose transport in the osteoblast and determine the role of the osteoblast glucose transporter4 in global glucose utilization. Finally, to test the validity of putative bone pancreas endocrine loop in vivo, we will determine the effect of a hyperinsulinemic euglycemic clamp on circulating undercarboxylated OC levels in both wild type and mice lacking Glut4 in osteoblasts. In essence, these studies will test the novel hypothesis that the osteoblast plays a critical role in the maintenance of glucose homeostasis. Disruption of Glut4 transporter in osteoblasts in vitro eliminated insulin-stimulated glucose uptake, reduced proliferation and diminished measures of osteoblast maturation. Glut4 Ob-KO mice exhibited increased peripheral adiposity in association with mild hyper-insulinemia but marked insulin resistance measured by ITT. Applicable research area(s): Metabolism
Status	PhenStat Analysis Verified
Public Release	12/10/2017
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Type	Count
Animals	14
Experimental Conditions	1
Catalog Items	3
Curation Info (# flags)	2
Phenotype Assays	13
Phenotype Measurements	182
Histology Images	0
Publications	0

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	0	6	0
B6.129-Slc2a4^tm1Abel Tg(BGLAP-cre)1Clem	Glut4^lox/lox Tg(BGLAP-cre)1Clem	0	8	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor (Units)
Experimental Group

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
body mass	W rodent
fat body mass	fat body mass
lean body mass	lean body mass
glucose infusion rates	glucose infusion rates
glucose, basal	glucose, basal
hepatic glucose production, basal	hepatic glucose production, basal
glucose turnover, whole body	glucose turnover, whole body
glycogen/lipid synthesis, whole body	glycogen/lipid synthesis, whole body
glycolysis, whole body	glycolysis, whole body
glucose, clamp average	glucose, clamp average
Hepatic Glucose Production, clamp	Hepatic Glucose Production, clamp
glucose uptake, muscle	glucose uptake muscl
glucose uptake, white adipose	glucose uptake white

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