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Experiment

miR128 clamp
Summary Data Summary
Investigator Naar, Anders
Description miR128 LNA treatment may affect insulin sensitivity.

Lentiviruses encoding miR-128-1 (miRZip-128-1) and miR-148a (miRZip-148a)
precursors, as well as a scrambled control (miRZip-Scramble), were obtained from
System Biosciences, Inc. (no. CS970MZ-Z). 12-week-old male C57BL/6J mice were
purchased from the Jackson Laboratory, Bar Harbor, ME, and fed an HFD
supplemented with 60% kcal from milk fat (D12492, Research Diets) during
treatment. Mice were injected in the tail vein with 1 × 109 pfu/mouse diluted in
100 µl PBS on day 1, and serum and liver were harvested on day 14.

Our studies suggest that noncoding RNAs, such as miRNAs, uncovered in GWAS
analyses could play key roles in contributing to human disease states. The
therapeutic tractability of potent and specific antisense technologies targeting
single or multiple miRNAs implicated in human cardiometabolic diseases may thus
have important clinical ramifications for the treatment of these diseases.

Applicable research areas: Metabolism, Diabetes, Cardiovascular
Status Completed
Public Release 3/9/2018
Data Collected? Data will not be collected for this catalog item
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal CountExperiment Description
Data Analysis
TypeCount
Animals14
Experimental Conditions2
Catalog Items1
Curation Info (# flags)3
Phenotype Assays13
Phenotype Measurements182
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
14
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 miR128
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs.
 No Drug/Treatment
No Drug or Treatment done on these samples

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
Drug Administered
Experimental Group
Experimental Factor Values


No documents found.

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