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Measurement of energy expenditure, RER and body composition in 28 month old mice
on one of 3 diets (control (AIN93G), ketogenic, or low carbohydrate diets for 1
To measure energy expenditure and body composition in 28 month old male mice on
one of three diets control (AIN93G), ketogenic (90% fat, 10% protein; 0.7%
carbohydrate), or low carbohydrate (70% fat, 20% protein and 10% carbohydrate).
All animals are fed 11.2 kcal/day of their designated diet. No DEXA was
performed due to concern about anesthesia in these older animals since they were
needed for additional testing (tissues) after metabolic cage analysis.
Applicable research area(s): Obesity, Metabolism
month(s) post-natal (m)
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Curation Info (# flags)
Name / Abbreviation
Experimental Factor: Experimental Group
This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor: Mouse Diet
based on AIN93G; caloric density=3.95 kcal/g
Ketogenic diet (90% fat, 10% protein, 0.7% carbohydrate); caloric density= 6.53 kcal/g
low carbohydrate diet (70%fat, 20% protein, 10% carbohydrate); caloric density=5.61 kcal/g
Experimental Factor (Units)
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oxygen consumption (Light Period)
respiratory exchange ratio (Light Period)
heat (Light Period)
oxygen consumption (Dark Period)
carbon dioxide production Light Period
carbon dioxide production Dark Period
respiratory exchange ratio (Dark Period)
heat (Dark Period)
carbon dioxide production
respiratory exchange ratio
Activity 24h (total x-axis)
Activity 24h total x
Activity 24h (x-ambulatory)
Activity 24h (x-amb)
Activity 24h (total vertical z-axis)
Activity 24h total z
heat - 24h
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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