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Experiment

Sable Energy Balance #21814 8/28/15
Summary Data Summary
Investigator McGuinness, Owen
Description Energy expenditure measured by indirect calorimetry in the Promethion by Sable
Systems. Activity, food intake and water intake are also continuously measured.
Measurements were taken for 8 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.

Groups:
Male WT (C57BL/6J ) mice.
Mice were fed a chow diet (HarlanTD7012) or a High Fat Diet (BioServ F3282).
Mice were injected with either Saline or LPS (Lipopolysaccharide).
Male WT Saline Chow: n=4,
Male WT LPS Chow: n=4,
Male WT Saline HF: n=4,
Male WT LPS HF: n=4,

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure and feeding behaviors.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure, feeding behavior.
Status Completed
Public Release 4/7/2018
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals16
Experimental Conditions3
Catalog Items1
Curation Info (# flags)2
Phenotype Assays12
Phenotype Measurements192
Histology Images0
Publications0
Phenotypes2


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
16
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 LPS
Lipopolysaccharide - induces bacterial infection
 Saline
Saline

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 BioServ F3282
Rodent Hig Fat Diet. Protein 20.%, Fat 35.5%, Fiber 0.0%, Ash 3.2%, Moisture <5.0%, Carbohydrate 36.3%. Ingredients: Lard, Casein, Dextrose, Sucrose, DL-Methionine, Choline Chloride, Vitamin Mix and Mineral Mix
 Teklad - 7012
Crude Protein (Min) 19.0%, Crude Fat (Min) 5.0%, Crude Fiber (Max) 5.0%, Protein %19.92, Fat %5.67, Fiber %4.37, Ash %6.48, Ingredients - Ground corn, dehulled soybean meal, ground oats, wheat middlings, dehydrated alfalfa meal, soybean oil, corn...
Experimental Factor (Units)
Drug Administered
Experimental Group
Mouse Diet
Experimental Factor Values


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