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Experiment

Gut microbiota analysis: Effect of OGG1 knockout in Chow and HFD-fed mice
Summary Data Summary
Investigator Sampath, Harini
Description Male WT and Ogg1 -/- (OKO) (C57BL/6 background) mice were fed a standard chow
diet (Picolab Laboratory Rodent Diet (5L0D), Purina Mills) or high fat diet
(Research Diets 12492) for ~16 weeks. Starting at 12-14 weeks of age, animals
were individually housed for the diet studies. Fresh fecal samples were
collected after 5 weeks of feeding in individually housed setting (19-20 weeks
total) and sent for 16S rDNA amplicon sequencing analysis. Chow animals
previously characterized were reanalyzed with the HFD animals.

Applicable research area(s): Obesity, Metabolism
Status Completed
Public Release 6/10/2018
Animal Age Measured In: week(s) post-natal (w)
Data Analysis
TypeCount
Animals24
Experimental Conditions2
Catalog Items1
Curation Info (# flags)0
Phenotype Assays1
Phenotype Measurements24
Histology Images0
Publications0
External References2
Documents4


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
12
0
Ogg1 -/-
0
12
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet 5001
crude protein (23.0%), crude fat (4.5%), crude fiber (6.0%), Ash (< 8.0%), minerals (2.5%)
 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat
Experimental Factor (Units)
Experimental Group
Mouse Diet


Phenotype Assays Add / Edit



No documents found.

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