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Experiment

Liver TG measurements in B6 mice fed protein restricted diets
Summary Data Summary
Investigator Ryan, Karen
Description 64 mice (37 male, 27 female) age 13 weeks were placed on one of three diets
containing either 20%, 5%, or 1.25% protein. Prior to placing animals on trial
diets an NMR was performed to determine body composition. Body weight and feed
weight were weighed every 2 days to observe weight changes and food intake. At
days 14 and 28 NMR was performed to measure body composition and at day 23 a
glucose tolerance test was performed via IP injection. Sac for tissue (liver,
brain, plasma, WAT, BAT) was performed on day 30 (17 weeks old). Tissues were
flash frozen and stored at -80C.

Applicable research area(s): Obesity, Metabolism
Status Completed
Public Release 6/14/2018
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals64
Experimental Conditions5
Catalog Items2
Curation Info (# flags)1
Phenotype Assays2
Phenotype Measurements128
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
27
37
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fed
Animals are allowed to have food.

Experimental Factor: Mouse Diet

 Research Diets - D11051801
Rodent Diet with 22% fat, 60% carbohydrate, 18% protein
 Research Diets - D11092301
Rodent Diet with 22% fat, 74% carbohydrate, 4% protein
 Research Diets - D11092310
Rodent Diet with 22% fat, 77% carbohydrate, 1% protein

Experimental Factor: Tissue Source

 liver
liver


Phenotype Assays Add / Edit


No documents found.

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