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Experiment

Effect of Mecp2-e1 knockout (model of Rett syndrome) on energy expenditure and
meal patterns in male and female mice
Summary Data Summary
Investigator Yasui, Dag
Description Mutations in MECP2 cause the neurodevelopmental disorder Rett syndrome. The
Yassui/ LaSalle lab has generated a MeCP2-e1 mouse line. These MeCP2-e1
deficient mice developed forelimb stereotypy, hindlimb clasping, excessive
grooming and hypo-activity prior to death between 7 and 31 weeks. Interestingly
the female mice heterozygote (+/-) were found to gain weight at a significantly
greater rate than WT litter mates.

Therefore, we aimed to determine if the heterozygote female (+/-) or hemizygote
male (-/y) demonstrated different energy utilization when compared to littermate
controls (+/+ and +/y, respectively).

Indirect calorimetry measurements were completed over three days and animals
body composition determined by DEXA.

Applicable research area(s): Obesity, Metabolism, Neurobiology
Status Completed
Public Release 6/8/2019
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals40
Experimental Conditions2
Catalog Items3
Curation Info (# flags)1
Phenotype Assays49
Phenotype Measurements1960
Histology Images0
Publications0
Phenotypes18


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+
12
9
0
C57BL/6N-Mecp2tm1.1Dhy
0
8
0
C57BL/6N-Mecp2tm1.1Dhy -/+
11
0
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
 Experiment-Het
This animal belongs to the experimental group and is heterozygous wild type/mutation for the affected locus.

Experimental Factor: Mouse Diet

 LabDiet 5058
PicoLab Mouse Diet 20, 20.0% protein (Calories: 23% protein, 22% Fat, 55% Carbohydrate)
 Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...


Phenotype Assays Add / Edit

NameAbbreviation
body mass
W rodent
oxygen consumption (Light Period)
VO2-L
fat body mass
fat body mass
lean body mass
lean body mass
respiratory exchange ratio (Light Period)
RER-L
bone mineral density
BMD
bone mineral content
BMC
bone area
bone area
fat tissue, %
% body fat
heat (Light Period)
HEAT-Light
oxygen consumption (Dark Period)
VO2-D
carbon dioxide production Light Period
VCO2-L
carbon dioxide production Dark Period
VCO2-D
respiratory exchange ratio (Dark Period)
RER-D
heat (Dark Period)
HEAT-Dark
carbon dioxide production
VCO2
heat
HEAT
oxygen consumption
VO2
respiratory exchange ratio
RQ
energy intake (Light Period)
food intake - light
food intake (Dark Period)
food intake - dark
energy intake (avg 24 hr)
energy intake avg 24
energy intake (Dark Period)
energy intake - dark
Activity 24h (total x-axis)
Activity 24h total x
Activity 24h (x-ambulatory)
Activity 24h (x-amb)
Activity 24h (total vertical z-axis)
Activity 24h total z
tissue area
tissue area
total tissue mass
TTM
heat (24hr)
heat - 24h
meal duration (Avg) - Active Eating Time
avg meal dur AET
meal size (Avg)
avg meal size
meals, number
num meals
inter-meal interval, average
avg IMI
meal duration (total)
total meal duration
satiety ratio
satiety ratio
eating rate, average
avg eating rate
meal duration, average light
avg light-meal dur
meal duration, average dark
avg dark-meal dur
meal size, average light
avg light-meal size
meal size, average dark
avg dark-meal size
inter-meal interval, average light
avg light-IMI
inter-meal interval, average dark
avg dark-IMI
meals, number light
light-num meals
meals, number dark
dark-num meals
satiety ratio, light
light-satiety ratio
satiety ratio, dark
dark-satiety ratio
eating rate, light
light-eating rate
eating rate, dark
dark-eating rate
food intake
food intake



No documents found.


Mammalian Phenotype (MP) Terms

MPTermIDMPTermNameDefinitionOther Results
MP:0000063decreased bone mineral density
reduction in the quatitative measurment value of mineral content of bone; BMD is used as an indicator of bone strength used as a measure of structural strength and screen for osteoporosis; bone mineral density is the ratio of bone mineral content to bone size [ISBN:0-683-40008-8| MGI:csmith]
MMPC Search Results
MP:0001402hypoactivity
general reduced locomotor activity; reduced movement from one place to another or having a decreased state of activity [ISBN:0-683-40008-8| MGI:csmith]
MMPC Search Results
MP:0003961decreased lean body mass
reduced amount of the fat-free physical bulk or volume of the body including all its components except adipose (fat) tissue [MGI:csmith]
MMPC Search Results
MP:0005202lethargy
mild impairment of consciousness resulting in reduced alertness and awareness and/or sluggish behavior or inactivity; can be due to generalized brain dysfunction [ISBN:0-683-40008-8]
MMPC Search Results
MP:0005290decreased oxygen consumption
less than the normal rate at which oxygen enters the blood from alveolar gas; this is equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body [MESH:G06.535.723]
MMPC Search Results
MP:0010124decreased bone mineral content
reduction in the amount (usually in grams/cm) of bone mineral divided by a bone-scanned area [MGI:csmith]
MMPC Search Results
MP:0010378increased respiratory quotient
increase in the ratio of the volume of carbon dioxide released to oxygen consumed by a body tissue or an organism compared to controls [MGI:csmith]
MMPC Search Results
MP:0010968decreased compact bone area
reduction in the total amount of cross-sectional area of compact bone tissue [MGI:csmith]
MMPC Search Results
MP:0011939increased food intake
increase in the total number of calories/food amount taken in over time when compared to the normal state [MGI:csmith]
MMPC Search Results
MP:0011940decreased food intake
reduction in the total number of calories/food amount taken in over time when compared to the normal state [MGI:csmith]
MMPC Search Results
MP:0011945increased eating frequency
increase in the number of discrete instances of initiation of eating over time| regardless of amount eaten [MGI:csmith]
MMPC Search Results
MP:0011946decreased eating frequency
reduction in the number of discrete instances of initiation of eating over time| regardless of amount eaten [MGI:csmith]
MMPC Search Results
MP:0012344decreased gnawing activity
decreased biting or chewing on something [http://orcid.org/0000-0001-5208-3432]
MMPC Search Results
MP:0013616decreased volumetric bone mineral density
reduction in the mineral mass per unit volume of bone| the hard| rigid form of connective tissue constituting most of the skeleton of vertebrates and composed chiefly of calcium salts; this is expressed as the amount of mineral per cubic cm of bone (usually in mgHA/cm^3)| with results generated from Quantitative computed tomography (QCT) and other tests [PMID:12730800| PMID:17481978]
MMPC Search Results
MP:0013618decreased areal bone mineral density
reduction of the mineral mass per unit area of bone| the hard| rigid form of connective tissue constituting most of the skeleton of vertebrates and composed chiefly of calcium salts; expressed as the amount of mineral per area cm^2 of bone (usually in g/cm^2)| with results generated from Dual-energy X-ray absorptiometry (DXA or DEXA) tests [PMID:12730800| PMID:17481978]
MMPC Search Results
MP:0014025decreased subchondral bone mineral content
decreased amount (usually in grams/cm) of bone mineral divided by a bone-scanned area in the bone region of the epiphysis that provides underlying mechanical and physiological support for the cartilage of joint articular surfaces [MGI:csmith| PMID:20392241]
MMPC Search Results
MP:0020010decreased bone mineral density of femur
reduction in the quatitative measurment value of mineral content of bone in the long bone of the thigh [http://orcid.org/0000-0001-5208-3432]
MMPC Search Results
MP:0020211slow movement
decreased rate or speed of the coordinated movements executed with a purpose [http://orcid.org/0000-0001-5208-3432]
MMPC Search Results

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