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Experiment

NEXLPL+/- CLAMS
Summary Data Summary
Investigator Eckel, Robert
Description Free fatty acids (FFAs) suppress appetite when injected into the hypothalamus.
To examine whether lipoprotein lipase (LPL), a serine hydrolase that releases
FFAs from circulating triglyceride (TG)-rich lipoproteins, might contribute to
FFA-mediated signaling in the brain, we created neuron-specific LPL-deficient
mice. Homozygous mutant (NEXLPL-/-) mice were hyperphagic and became obese by 16
weeks of age. These traits were accompanied by elevations in the hypothalamic
orexigenic neuropeptides, AgRP and NPY, and were followed by reductions in
metabolic rate. The uptake of TG-rich lipoprotein fatty acids was reduced in the
hypothalamus of 3-month-old NEXLPL-/- mice. Moreover, deficiencies in essential
fatty acids in the hypothalamus were evident by 3 months, with major
deficiencies of long-chain n-3 fatty acids by 12 months. These results indicate
that TG-rich lipoproteins are sensed in the brain by an LPL-dependent mechanism
and provide lipid signals for the central regulation of body weight and energy
balance.

Applicable research area(s): Diabetes, Obesity, Metabolism, Neurobiology
Status Completed
Public Release 7/13/2018
Data Collected? Data will not be collected for this catalog item
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal CountExperiment Description
Data Analysis
TypeCount
Animals16
Experimental Conditions1
Catalog Items1
Curation Info (# flags)3
Phenotype Assays17
Phenotype Measurements272
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
9
0
C57BL/6-Lpltm1Ijg Neurod6tm1(cre)Kan/+
0
7
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment-Het
This animal belongs to the experimental group and is heterozygous wild type/mutation for the affected locus.
Experimental Factor (Units)
Experimental Group
Experimental Factor Values


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