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Experiment

Circulating ALT levels in liver specific Nr5a2 KO mice treated with AAV8-Lrh1
Summary Data Summary
Investigator Ingraham, Holly
Description This Lrh-1 liver specific knockout is a model of non-alcoholic fatty liver
disease

8 week old male mice were placed on a high fat diet (Research Diets 12492) for 6
weeks. Mice had adlib access to food and water. Mice were fasted for 6 hours
prior to blood collection. Mice were anesthesized with avertin prior to
collecting blood via sub-mandibular bleed.

These knockouts were generated by infecting Lrh-1 Floxed mice with
adeno-associated virus 8 (AAV8) expressing Cre recombinase (control mice). hLrh1
mice were generated by coinfecting Lrh-1 floxed mice with AAV8 expressing Cre
and AAV8 expressing hLrh1 (experiment mice). AAV8 concentration was 1x10^11
gc/mL for Cre and 5x10^10 for hLrh1.

Applicable research area(s): Diabetes, Metabolism
Status Completed
Public Release 8/4/2019
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals16
Experimental Conditions8
Catalog Items1
Curation Info (# flags)1
Phenotype Assays1
Phenotype Measurements24
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
Nr5a2 LKO
0
16
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)

Experimental Factor: Viral Dose Units

 GC/ml
genome copies per milliliter

Experimental Factor: Virus Injected

 AAV8-Cre
AAV serotype 8 virus expresses Cre Recombinase
 AAV8-Cre-GFP
AAV serotype 8 virus expresses Cre Recombinase and eGFP marker
 AAV8-Lrh1
AAV serotype 8 virus expresses Lrh1
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

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