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Experiment

Circulating ALT and FFA levels in liver specific Nr5a2 KO mice Part 2
Summary Data Summary
Investigator Ingraham, Holly
Description This Lrh-1 liver specific knockout is a model of non-alcoholic fatty liver
disease

8 week old male mice were placed on a high fat diet (Research Diets 12492) for 6
weeks. Mice had adlib access to food and water. Mice were fasted for 6 hours
prior to blood collection. Mice were anesthesized with avertin prior to
collecting blood via sub-mandibular bleed.

These LRH-1flox/flox mutant mice possess loxP sites flanking exon 5, encoding
the second zinc finger of the DNA-binding domain of the nuclear receptor
subfamily 5, group A, member 2 (Nr5a2) gene. LRH-1 is a transcription factor
expressed in liver, pancreas, intestine, and ovary where it regulates
cholesterol and bile acid homeostasis, cell proliferation, and steroidogenesis.
Homozygous mice are viable and fertile. When bred to mice that express
tissue-specific Cre recombinase, resulting offspring will have exon 5 deleted in
the cre-expressing tissues.
Status Completed
Public Release 8/4/2019
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals12
Experimental Conditions5
Catalog Items2
Curation Info (# flags)2
Phenotype Assays2
Phenotype Measurements24
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
Nr5a2 LKO
0
12
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 LabDiet 5053
PicoLab Rodent Diet 20, 21.0% protein (Calories: 24.5% protein, 13.1% Fat, 62.4% Carbohydrate)
 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)
Experimental Factor Values


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