mmpc-logo mmpc-logo
twitter-logo @NationalMMPC
| Create Account | login
Experiment

Experiment for Order #1642
Summary Data Summary
Investigator Huen, Sarah
Description Macrophage infiltration is a prominent feature of the innate immune response to
kidney injury. The persistence of macrophages is associated with
tubulointerstitial fibrosis and progression of chronic kidney disease.
Macrophages are known to be major producers of transforming growth factor-ß1
(TGF-ß1), especially in the setting of phagocytosis of apoptotic cells. TGF-ß1
has long been implicated as a central mediator of tissue scarring and fibrosis
in many organ disease models, including kidney disease. In this study, we show
that homozygous deletion of Tgfb1 in myeloid lineage cells in mice heterozygous
for Tgfb1 significantly reduces kidney Tgfb1 mRNA expression and Smad activation
at late time points after renal ischemia-reperfusion injury. However, this
reduction in kidney Tgfb1 expression and signaling results in only a modest
reduction of isolated fibrosis markers and does not lead to decreased
interstitial fibrosis in either ischemic or obstructive injury models. Thus,
targeting macrophage-derived TGF-ß1 does not appear to be an effective therapy
for attenuating progressive renal fibrosis after kidney injury.

Applicable research area(s): Genitourinary
Status Completed
Public Release 5/21/2011
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainMetadataExperiment Description
Data Analysis
TypeCount
Animals35
Experimental Conditions1
Catalog Items1
Curation Info (# flags)3
Phenotype Assays1
Phenotype Measurements34
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
22
0
LysM-Cre;Tgfb f/n
0
13
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
Experimental Group
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.