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Strain
C57BL/6-Tgfb1tm2Flv Lyz2tm1(cre)Ifo

Summary Data Summary
Official Name C57BL/6-Tgfb1tm2Flv Lyz2tm1(cre)Ifo
Common Name LysM-Cre;Tgfb f/n
Description macrophage-specific TGFb1 knockout

LysM-Cre mice (Jackson Laboratory, Bar Harbor, ME) were bred
with Tgfb1f/n mice (gift from Dr. Richard Flavell)

Tgfb1 KO: A green-fluorescent protein was inserted into the
first exon of the gene to generate a null allele. A floxed
neo cassette inserted downstream of the modified exon 1 was
removed by cre-mediated recombination.

LysMCre: An NLS-cre gene was inserted into the endogenous
ATG start site of the gene. An frt-flanked neomycin cassette
was removed in ES cells via Flp-mediated recombination prior
to production of chimeric mice.
Development Status Phenotyping ongoing
Creation Method transgenic
Breeding Type backcross
TypeCount
Investigators 1
Genomics - Modifications 2
Genomics - Transgenes 1
Experiments 2


Investigators
NameInstitution
Sarah HuenYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Tgfb1knockoutknockoutNot Specified
Lyz2knockin (Cre)wildNot Specified
EnhancerPromoterGeneCopies
Not SpecifiedLyz2CRENot Specified


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