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Experiment

Circulating insulin levels in B6 mice gavaged with derivatives of niclosamide
(part 3)
Summary Data Summary
Investigator Jin, Shengkan
Description 5 hour-fastecd C57BL/6J mice were orally gavaged with vehicle (solution contains
2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin) or individual drug
suspension of niclosamide derivatives. Blood was collected through tail vein
before and after oral gavage with heparin-coated tube. Plasma was obtained by
centrifugation at 1000g for 10-15min at 4? , and stored at -80? for further
analysis.

Niclosamdie and its derivatives are a group of mitochondrial uncouplers that can
stimulates oxygen consumption.

Hypothesis: Acute oral administration of mitochondrial uncouplers could increase
insulin sensitivity.

The objective of the study is to investigate the acute effect of mitochondrial
uncoupler on metabolomics and insulin sensitivity.

Applicable research area(s): Diabetes, Endocrine deficiency, Metabolism
Status Completed
Public Release 4/6/2020
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals18
Experimental Conditions9
Catalog Items1
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements36
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
18
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 MB1-105
MB1-105, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
 MB1-98
MB1-98, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
 MB5-6
MB5-6, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
 No Drug/Treatment
No Drug or Treatment done on these samples
 Vehicle
Vehicle - Control
 WX06
WX06, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution
 Y05
Y05, a niclosamide derivative, in 2% DMSO, 10% solutol, 88% of 20% beta-cyclodextrin solution

Experimental Factor: Drug Delivery Route

 PO
The drug was delivered orally

Experimental Factor: Drug Dose Units

 mg/kg
milligrams per kilogram

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 LabDiet 5001
crude protein (23.0%), crude fat (4.5%), crude fiber (6.0%), Ash (< 8.0%), minerals (2.5%)

Experimental Factor: Tissue Source

 plasma (lithium heparin)
plasma (lithium heparin)
Experimental Factor Values


Phenotype Assays Add / Edit


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