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Experiment

NMR of DIO C57BL/6 and Control Mice

Summary

Data Summary

Investigator	Jones, Graham
Description	These mice are part of a study aimed at evaluating the impact of body composition on motivated feeding, specifically the lasting effects of having a history of obesity and then subsequently losing weight. These mice are all genetically wild type C57BL6 animals, and represent the diet-induced obesity (DIO) leg of the studies. There are 3 groups of animals with both males (M) and females (F) comprising each group. The control animals (DIOCM and DIOCF) have been maintained on normal chow for the entirety of their lives. The DIO (DIOM and DIOF) and DIO-weight loss (DIOWLM and DIOWLF) animals were weaned onto a high-fat (36%) high-sucrose (30%) (HFHS) chow to stimulate weight gain, predominately through the accrual of adipose tissue. After reaching a body weight criteria (35 g for F, and 45 g for M) the DIOWL group was switched to ad libitum normal chow to induce weight loss, additional calorie restriction was used to return body weights down to the level of the DIOC mice; the DIO animals stayed on the HFHS chow. The mice were then assayed in operant paradigms to assess motivated feeding. The NMR was conducted following all behavioral testing and after the animals had been housed with ad libitum access to their respective diets (normal chow for DIOC and DIOWL groups, and HFHS chow for DIO groups) for at least two weeks. Applicable research area(s): Obesity, Metabolism
Status	Completed
Public Release	5/22/2020
Animal Age	Measured In: week(s) post-natal (w)
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Data Analysis

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Type	Count
Animals	51
Experimental Conditions	2
Catalog Items	1
Curation Info (# flags)	4
Phenotype Assays	4
Phenotype Measurements	204
Histology Images	0
Publications	0
Phenotypes	5

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6	C57BL/6	28	23	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	BioServ F0071	Protein 18.7%, Fat 5.6%, Fiber 4.7%, Ash 6.5%, Moisture <10%, Carbohydrate 59.1%; Calories: 3.6 kcal/gm
	LabDiet PicoLab Rodent Diet 5LOD	Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...

Experimental Factor (Units)
Experimental Group
Mouse Diet

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
fat body mass	fat body mass
lean body mass	lean body mass
total body weight	total body weight
body water	body fluid

No documents found.

Mammalian Phenotype (MP) Terms

MPTermID	MPTermName	Definition	Other Results
MP:0000679	increased percent water in carcass	more than the normal total amount of water retained in the body measured post mortem [MGI:il]	MMPC Search Results
MP:0001260	increased body weight	greater than normal average weight [J:33400]	MMPC Search Results
MP:0003960	increased lean body mass	greater amount of the fat-free physical bulk or volume of the body including all its components except adipose (fat) tissue [MGI:csmith]	MMPC Search Results
MP:0010024	increased total body fat amount	greater than the normal total amount of connective tissue composed of fat cells within the entire body [MGI:csmith]	MMPC Search Results
MP:0014142	increased body fat mass	increased physical bulk or volume of fat in the whole body [MGI:csmith]	MMPC Search Results

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