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Influence of Myo K64A mutation on metabolic phenotype and energy balance
Summary Data Summary
Investigator Adams, Sean
Description Oxygenated myoglobin has been shown to bind fatty acids or fatty acylcarnitines,
where the Lysine at position 64 is critical to enable binding of fatty acids and
fatty acylcarnitines. However, it is unknown if myoglobin is involved in
whole-body fatty acid oxidation. The objective of this study is to determine if
knocking out myoglobin or inducing a K64A point mutation to prevent lipid
binding influences substrate oxidation (respiratory exchange ratio) and other
metabolic phenotypes in high-fat fed, cold-challenged mice.

Male & female MyoK64A and litter mate controls will have their baseline energy
expenditure assessed by CLAMS at 5 weeks of age. Animals will be placed on
high-fat (HFD) diet at 6 weeks After 5 weeks on diet (age= 11 w) and 12 weeks on
diet (age=17 w) energy expenditure will be reassessed. Energy expenditure will
be assesses at 10 and 22°C followed by body composition analysis by DEXA.
Status Completed
Public Release 2/21/2022
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
Experimental Conditions4
Catalog Items7
Curation Info (# flags)2
Phenotype Assays31
Phenotype Measurements7440
Histology Images0

Strain NameCommon NameFemalesMalesUnknown
C57BL/6N +/+

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Environmental Temperature (°C)

 env temp
The temperature of the environment when the measurement was taken.

Experimental Factor: Experimental Group

This animal belongs to the control group for the experiment.
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Teklad - 08511
45% Fat Kcal Diet (ISP) -- The mid-fat diet in a series of diets with isolated soy protein, 10, 45, or 60% kcal from fat, with ~43% of the total carbohydrate from sucrose, and equivalent vitamin and mineral levels adjusted on an energy basis....
 Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...

Experimental Factor: Total Time on Diet (wk)

 total time on diet
The total amount of time, in weeks, the subject spent on the particular diet.

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