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Experiment

Influence of single meal on energy expenditure and substrate oxidation in PKD
mice.
Summary Data Summary
Investigator Weimbs, Thomas
Description Autosomal dominant polycystic kidney disease (ADPKD) is characteristized by the
gradual replacement of normal renal parenchyma by cysts, which culminates in
renal failure in approximately 50%of patients. Previous studies indicate that
reduced food intake is effective in suppressing PKD disease progression in mice.
However, it is unknown if this is due to a whole-body shift in substrate
oxidation. The objective of this study is to determine the influence of single
meal and fasting on the substrate oxidation (respiratory exchange ratio) of PKD
mouse (Pkd1cond/cond:Nestincre) compared to wild type (C57BL/6) mice.
Energy expenditure, physical activity, and food intake were evaluated in 7 male
mice (2 WT and 5 PDK mice) fed powdered chow diet (PicoLab 20) by indirect
respiratory calorimetry in the CLAMS (Comprehensive Lab Animal Monitoring
System, Columbus Instruments). Animals were acclimated to the facility for at
least 1 week prior to initiation of single meal feeding. Animals were provided
~6g (20.46 kcal) in a single ad lib meal at ~ 8 AM each day for a week prior
CLAMS at 8 or 12 weeks of age. Animals were acclimated to the CLAMS cages for 48
hours and to the light and temperature-controlled chamber for 24 hours prior to
testing. Food access was restricted to 8 hrs during the light cycle, 8AM to ~4PM
during the 24 hours prior to testing and during testing (exception # 78.8,
restricted access for only the last 24 hr at 8 week). Analyzed data constitutes
data collected from 48 hours of continuous measurement (2 light/2 dark cycles).
Oxygen consumption and carbon dioxide production were measured and used to
calculate energy expenditure (or heat production, kilocalories (kcal)) and
respiratory exchange ratio (RER: VCO2/VO2). Cage-mounted sensors detect and
record measurements of physical activity, food intake and water intake.
Status Completed
Public Release 8/30/2021
Animal Age Measured In: week(s) post-natal (w)
Data Analysis
TypeCount
Animals12
Experimental Conditions2
Catalog Items3
Curation Info (# flags)0
Phenotype Assays31
Phenotype Measurements744
Histology Images0
Publications0
Phenotypes5


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+
5
2
0
Pkd1tm2Ggg Tg(Nes-cre)Wme
0
5
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 20
a regular chow diet comprising 23.6% protein, 11.9% fat, and 64.5% carbohydrates (PicoLab Rodent Diet 20, LabDiet)
Experimental Factor (Units)
Experimental Group
Mouse Diet



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