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Experiment

Hyperinsulinemic Clamp Order #1848 2/11/10
Summary Data Summary
Investigator Gettys, Thomas W
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups: WT

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.
Status Completed
Public Release 3/9/2012
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals17
Experimental Conditions4
Catalog Items1
Curation Info (# flags)2
Phenotype Assays17
Phenotype Measurements816
Histology Images0
Publications0
Phenotypes5


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
17
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 methionine restricted diet (0.17%)
Methionine restricted diet - 0.17% methionine, glutamic acid 3.39%, energy content 3.812 kcal/g, 18.9% of calories from fat, 64.9% carbohydrate, and 14.8% a custom mix of L-amino acid.
 methionine restricted diet (0.86%)
Methionine restricted diet - 0.86% methionine, L-glutamic acid 2.7%, energy content 3.812 kcal/g, 18.9% of calories from fat, 64.9% carbohydrate, and 14.8% a custom mix of L-amino acid.
Experimental Factor Values


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