MMPC :: Experiment

Experiment

Circulating insulin levels following an OGTT in B6 mice fed high fat diet and
treated with human milk oligosaccharides

Summary

Data Summary

Investigator	Lee, Sunhye
Description	The objective of this study was to investigate the beneficial effects of HMOs on metabolic and cognitive functions in high fat fed mice. Mice (6 weeks old) were acclimated for 1 week and divided into 4 groups as follows, and fed individual diets for 8 weeks. HMOs were provided in diets. 4 groups 10% low fat 10% low fat + 10% HMOs 45% high fat, 17% Sucrose 45% high fat, 17% Sucrose + 10% HMOs After 6-hour fasting, an OGTT was performed and samples were collected 15 minutes after glucose gavage. Plasma was collected using heparinized tubes and tissues (liver, visceral fats, small intestines, colon, cecum contents, brain, nodose ganglion) were collected for analysis. Applicable research areas: Metabolism, Obesity
Status	Completed
Public Release	8/27/2021
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6J	C57BL/6J	0	40	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	HMO	Human milk oligosaccharides
	No Drug/Treatment	No Drug or Treatment done on these samples
	Experimental Factor: Drug Delivery Route
	Drinking Water	The drug was delivered in the drinking water.
	Experimental Factor: Drug Dose Units
	%	% of drug in vehicle
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Feeding Group
	Fasted	Animals are deprived of food but not water.
	Fed	Animals are allowed to have food.
	Experimental Factor: Mouse Diet
	Research Diets - D12450J	Rodent Diet with 10% fat, 70% carbohydrate, 20% protein
	Research Diets - D12451	Research Diets - D12451 Rodent Diet with 45% kcal% fat
	Experimental Factor: Tissue Source
	plasma (lithium heparin)	plasma (lithium heparin)

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
insulin, plasma	insulin, plasma

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