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Experiment

Experiment for Order #1785
Summary Data Summary
Investigator Veron, Delma
Description We generated tetracycline-regulated transgenic mice (podocin-rtTA:tet-O-VEGF164)
that overexpress VEGF-A in podocytes upon induction with doxycycline by breeding
podocin-rtTA mice16 with tet-O-VEGF164 mice,17 which carry FVB background.
Transgenic mice were genotyped by reverse transcriptase-PCR using reported
primers.16,17 Adult podocin-rtTA:tet-O-VEGF164 mice (2–3 months old) received
doxycycline (0.625 mg/g chow, Harlen-Teklar, Madison, WI, USA) for 1 month (TOPO
+dox, n=14). Uninduced podocin-rtTA:tet-O-VEGF164 (TOPO -dox, n=9), induced
single transgenics (CON +dox, n=4), and uninduced single transgenics (CON -dox,
n=4) served as controls. Additional podocin-rtTA:tet-O-VEGF164 mice were induced
with doxycycline for 1 month, and then switched to regular diet for 2 weeks to
examine the reversibility of VEGF164-induced changes (TOPO ±dox, n=6).

We sought to examine the pathogenic role of excessive VEGF-A expression in
podocytes, since it has been reported that diabetic nephropathy and other
glomerular diseases are associated with increased VEGF-A expression. The
induction of podocyte-specific VEGF164 overexpression in adult transgenic mice
led to proteinuria, glomerulomegaly, glomerular basement membrane thickening,
mesangial expansion, loss of slit diaphragms, and podocyte effacement. When
doxycycline-mediated VEGF164 was stopped, these abnormalities reversed. These
findings were associated with reversible downregulation of metalloproteinase 9
and nephrin expression.

Applicable research area(s): Diabetes, Developmental biology
Status Completed
Public Release 5/20/2012
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainMetadataExperiment Description
Data Analysis
TypeCount
Animals9
Experimental Conditions1
Catalog Items1
Curation Info (# flags)3
Phenotype Assays1
Phenotype Measurements9
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
FVB/NJ
2
3
0
TOPO
2
2
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
Experimental Group
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

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