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Experiment

Liver lipid levels in Myc KO mice fed high fat and high cholesterol diet
Summary Data Summary
Investigator Rodrigues, Claudia
Description Endothelial Dysfunction. To investigate the role of endothelial c-Myc in tissue
homeostasis and identify potential therapeutic targets for cardiovascular
disease

For the FVB/N-Tg(tetO-MYC)36aBop/J animals, doxycycline was withdrawn at 4 weeks
and the experimental diet started at 8 weeks of age. For the
B6.129S6-Myctm2Fwa/Mmjax strain, at 4 weeks of age, the animals were injected
intraperitoneally with tamoxifen for 5 consecutive days. The experimental diet
started at 7 weeks. All the animals were put on the experimental diet for 5
weeks. Then the animals were fasted for 6 hours and anesthetized
intraperitoneally with a ketamine-xylazine solution. Blood was collected by
cardiac puncture in EDTA-coated tubes. Liver was harvested, snap frozen with
liquid N2 and then stored at -80 degree.
Status Completed
Public Release 3/26/2022
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainNo dataInconsistent Meta
Data Analysis
TypeCount
Animals35
Experimental Conditions5
Catalog Items1
Curation Info (# flags)3
Phenotype Assays2
Phenotype Measurements70
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
FVB/N +/+
0
13
0
FVB/N-Tg(tetO-MYC)36aBop/J
0
13
0
B6.129S6-Myctm2Fwa/Mmjax
0
4
0
B6.CBA-Tg(Cdh5-cre/ERT2)1Rha
0
5
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fed
Animals are allowed to have food.

Experimental Factor: Mouse Diet

 Teklad - 02028
Modification of TD.88137, to increase added cholesterol to 1.25% (total in the diet is 1.3%) and add 0.5% cholic acid, 15.4% kcal Protein, 41.9% kcal Carb, 42.6% kcal Fat
 Teklad - 08485
Harlan Teklad TD.08485 Low Fat Control Diet Purified Diet, Modification of TD.88137, Low Fat & No Added Cholesterol, Reduced Sucrose.

Experimental Factor: Tissue Source

 liver
liver


Phenotype Assays Add / Edit


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