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Experiment

Circulating corticosterone levels in B6 mice injected with MCMV and treated with
rat IgG, anti-NK1.1, anti-ASGM1
Summary Data Summary
Investigator Murphy, William
Description GVHD 50: compare the oucomes of diet-induced obese mice and control lean mice
after hematopoietic transplantation.

MCMV_Nkdep#2: checking cortecosterone of mice treated with/without NK cell
depletion after MCMV infection.

Tumor study 23: Investigating impact of tumor challenge on corticosterone across
sex and age of Lean/DIO mice.

We use anti-NK1.1 to deplete natural killer (NK) cells, anti-ASGM1 to deplete NK
and T cells, anti-PD-1 is the checkpoint blockade to increase the anti-tumor
effect, and the IgG is the isotype control for aPD-1.

Applicable research area(s): Obesity
Status Completed
Public Release 6/16/2022
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals15
Experimental Conditions12
Catalog Items1
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements15
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
15
0
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 anti-ASGM1
antibody to ASGM1
 anti-NK1.1
antibody to NK1.1
 rat IgG
rat immunoglobulin antibody

Experimental Factor: Drug Delivery Route

 IP
The drug was delivered intraperitoneal.

Experimental Factor: Drug Dose Units

 µg once
micrograms one dose
 µl once
microliters - one dose

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fed
Animals are allowed to have food.

Experimental Factor: Mouse Diet

 Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)

Experimental Factor: Viral Dose Units

 PFU
plaque forming units

Experimental Factor: Virus Injected

 MCMV
Mouse cytomegalovirus
 Vehicle
Injection of vehicle without virus. Negative control.
Experimental Factor Values


Phenotype Assays Add / Edit


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