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Experiment

Circulating lipid levels levels in Myc KO mice fed high fat and high cholesterol
diet Pt2
Summary Data Summary
Investigator Rodrigues, Claudia
Description At 5 weeks of age, the animals were injected intraperitoneally with tamoxifen
for 5 consecutive days. Diet treatment started at 2 weeks after tanmoxifen
injection. At endpoint, the animals were fasted for 6 hours and anesthetized
intraperitoneally with a ketamine-xylazine solution. Blood samples were
collected through cardiac puncture in EDTA-coated tubes. Plasma samples were
separated and stored at -80°C.
Status Completed
Public Release 12/10/2022
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainNo dataInconsistent MetaMouse Diet
Data Analysis
TypeCount
Animals77
Experimental Conditions5
Catalog Items5
Curation Info (# flags)4
Phenotype Assays3
Phenotype Measurements231
Histology Images0
Publications0
Phenotypes1


Animals
Strain NameCommon NameFemalesMalesUnknown
B6.129S6-Myctm2Fwa/Mmjax
22
14
0
B6.CBA-Tg(Cdh5-cre/ERT2)1Rha
18
23
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Teklad - 02028
Modification of TD.88137, to increase added cholesterol to 1.25% (total in the diet is 1.3%) and add 0.5% cholic acid, 15.4% kcal Protein, 41.9% kcal Carb, 42.6% kcal Fat
 Teklad - 08485
Harlan Teklad TD.08485 Low Fat Control Diet Purified Diet, Modification of TD.88137, Low Fat & No Added Cholesterol, Reduced Sucrose.
 Teklad - 88137
Harlan Teklad TD.88137 (Western Diet)

Experimental Factor: Tissue Source

 plasma (EDTA)
plasma (EDTA)
Experimental Factor Values


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