MMPC :: Experiment

Experiment

Effect of high glycemic index diet + sHE inhibitor trans-AUCB or HGI-curcumin on
open field performance in C57BL/6J mice

Summary

Data Summary

Investigator	Villablanca, Amparo
Description	Male and female C57BL/6J mice (~20 weeks) were place on a control (TD.08485) or HGI diet (TD.05230) and treated with the sHE inhibitor, trans-AUCB, via drinking water (1%w/v + PEG). In addition, a second group of animals were placed on a HGI diet + curcumin (polyphenol) (TD. 190668). Diet was provided for ~3 months prior to assessing motor function and anxiety by open field test. Mice, adapted to testing room for 60 min, were placed in the center of a white plastic box (40 x 40x 40 cm) and were tracked with an overhead camera for 20 min. Distance traveled and time spent in the perimeter (outside 10 cm) vs center (inside 30 cm) was determined.
Status	Completed
Public Release	12/14/2022
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6J	C57BL/6J	14	21	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	Soluble epoxide hydrolase (sEH) inhibitor	Soluble epoxide hydrolase (sEH) is an enzyme that catalyzes the hydrolysis of epoxyeicosatrienoic acids (EETs), which are lipid mediators derived from arachidonic acid through the cytochrome P450 epoxygenase pathway. Inhibitors sEH appear to reduce the
	Experimental Factor: Drug Delivery Route
	Drinking Water	The drug was delivered in the drinking water.
	Experimental Factor: Drug Dose Units
	%	% of drug in vehicle
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	Teklad - 05230	This is modified from TD 88137 to reduce fat content (replaced with cornstarch and maltodextrin). % kcal from Protein 18.7, Carbohydrate 68.7, and Fat 12.6. Total Kcal/g is 3.7
	Teklad - 08485	Harlan Teklad TD.08485 Low Fat Control Diet Purified Diet, Modification of TD.88137, Low Fat & No Added Cholesterol, Reduced Sucrose.
	Teklad TD.190668	18.65% protein, 68.7% carbohydrate, and 12.6% fat as percent kcal, with 34% sucrose and 0.05% curcumin by weight
	Experimental Factor: Open Field Zone
	Center	Animal was in the center of the open field zone of the arena.
	Perimeter	Animal was in the perimeter of the open field in the arena.

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
Time in Open Field Zone	Time in OPZ
Distance in Open Field Zone	Distance in OFZ

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