MMPC :: Experiment

Experiment

Effect of Lacto-N-tetraose, (LNT) on open field performance with control or high
fat diet

Summary

Data Summary

Investigator	Raybould, Helen
Description	40 mice, 8 wk old C57BL/6J (from Jax west room RB07) were fed either an AIN93G low fat diet (LF, 10%kcal fat/20%kcal protein/70%kcal CHO with 7% kcal sucrose), a high fat control diet (HF, 45%kcal fat/20%kcal protein/35%kcal CHO with 17% kcal sucrose) or a high fat diet with 10% (by mass) undigestable milk oligosaccharide Lacto-N-tetraose, LNT, (HF+LNT, 45%kcal fat/20%kcal protein/35%kcal CHO with 17% kcal sucrose + 10% LNT) for 8 weeks. Animals were divided into 2 cohorts of 20 animals for open field testing For this, mice are adapted to testing room for 60 min, were placed in the center of a white plastic box (40 x 40x 40 cm) and were tracked with an overhead camera for 15 min. Distance traveled and time spent in the perimeter (outside 10 cm) vs center (inside 30 cm) was determined.
Status	Completed
Public Release	2/9/2024
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

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Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6J	C57BL/6J	0	40	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Drug Administered
	lactose-N-tetraose	Lacto-N-tetraose is a complex sugar found in human milk. It is one of the few characterized human milk oligosaccharides (HMOs) and is enzymatically synthesized from the substrate lactose. It is biologically relevant in the early development of the infant
	Experimental Factor: Drug Delivery Route
	Diet	The drug was delivered via food.
	Experimental Factor: Drug Dose Units
	%	% of drug in vehicle
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	AIN-93G (10%F)	Low fat diet control based on BioServ AIN- 93G 10%kcal fat, 20%kcal protein, 70%kcal CHO with 7% kcal sucrose
	AIN-93G (45%F)	High fat diet based on BioServ AIN- 93G 45%kcal fat, 20%kcal protein, 35%kcal CHO with 17% kcal sucrose
	Experimental Factor: Open Field Zone
	Center	Animal was in the center of the open field zone of the arena.
	Perimeter	Animal was in the perimeter of the open field in the arena.

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
Time in Open Field Zone	Time in OPZ
Distance in Open Field Zone	Distance in OFZ

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