MMPC :: Experiment

Experiment

Circulating lipid, glucose and insulin, inflammatory marker, IGF1 and FGF21
levels in B6 mice fed ketogenic diet

Summary

Data Summary

Investigator	Ramsey, Jon
Description	At 18 months of age, male C57BL/6J mice were randomly assigned to the control (CD), intermittent ketogenic (IKD), or ketogenic (KD) diet. IKD mice were fed KD meals on three consecutive days of a week and the KD mice were fed a KD meal each day. The control diet contained (% of total kcal) 10% protein, 74% carbohydrate, and 16% fat. The ketogenic diet contained 10% protein, <0.5% carbohydrate, and 89.5% fat. At 27 months of age, mice were sacrificed with CO2 inhalation in a euthanasia chamber after an overnight fast (18 hours). Blood was collected with cardiac puncture. Serum was obtained and stored at -80 degree C. Liver tissues were snap frozen and stored in liquid nitrogen till analysis.
Status	Completed
Public Release	6/21/2023
Animal Age	Measured In: week(s) post-natal (w)
Flags

Data Analysis

DOWNLOAD ALL DATA (.csv)

Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6J	C57BL/6J	0	30	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Feeding Group
	Fasted	Animals are deprived of food but not water.
	Experimental Factor: Mouse Diet
	JJR_KETO	Ketogenic diet (90% fat, 10% protein, 0.7% carbohydrate); caloric density= 6.53 kcal/g
	JJR_Low Protein	Low protein(15% fat, 10% protein, 75% carbohydrate); caloric density= 3.77 kcal/g
	Experimental Factor: Tissue Source
	serum	serum

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
cholesterol, total	TC
insulin, plasma	insulin, plasma
triglycerides	TG
non-esterified free fatty acids	NEFA
glucose, plasma	plasma glucose
cholesterol, HDL, plasma	HDL-cholesterol
Interleukin 10	IL-10
Interleukin 1 beta	IL-1ß
Interleukin 6	IL-6
Interleukin 8 homologue	KC
Tumor necrosis factor alpha	TNFa
cholesterol, LDL and VLDL	LDL/VLDL
Insulin-like Growth Factor-1	IGF-1
Fibroblast growth factor 21	FGF-21

No documents found.

Mammalian Phenotype (MP) Terms

MPTermID	MPTermName	Definition	Other Results
MP:0001556	increased circulating HDL cholesterol level	higher amount in the blood of the small lipoprotein:cholesterol complex that transports cholesterol out of the arteries and to the liver for reprocessing or excretion [MGI:csmith]	MMPC Search Results
MP:0005178	increased circulating cholesterol level	greater than the normal amount in the blood of the principal sterol of vertebrates and the precursor of many steroids\| including bile acids and steroid hormones; it is a component of the plasma membrane lipid bilayer and of plasma lipoproteins and can be found in all animal tissues [MGI:csmith]	MMPC Search Results
MP:0005559	increased circulating glucose level	greater than the normal concentration in the blood of this major monosaccharide of the body; it is an important energy source [MGI:cwg\| RGD:cur]	MMPC Search Results

Add Experiment Strain Metadata