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Experiment

Circulating lipid, glucose and insulin, inflammatory marker, IGF1 and FGF21
levels in B6 mice fed ketogenic diet
Summary Data Summary
Investigator Ramsey, Jon
Description At 18 months of age, male C57BL/6J mice were randomly assigned to the control
(CD), intermittent ketogenic (IKD), or ketogenic (KD) diet. IKD mice were fed KD
meals on three consecutive days of a week and the KD mice were fed a KD meal
each day. The control diet contained (% of total kcal) 10% protein, 74%
carbohydrate, and 16% fat. The ketogenic diet contained 10% protein, <0.5%
carbohydrate, and 89.5% fat. At 27 months of age, mice were sacrificed with CO2
inhalation in a euthanasia chamber after an overnight fast (18 hours). Blood was
collected with cardiac puncture. Serum was obtained and stored at -80 degree C.
Liver tissues were snap frozen and stored in liquid nitrogen till analysis.
Status Completed
Public Release 6/21/2023
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals30
Experimental Conditions5
Catalog Items9
Curation Info (# flags)2
Phenotype Assays14
Phenotype Measurements420
Histology Images0
Publications0
Phenotypes3


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
30
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 JJR_KETO
Ketogenic diet (90% fat, 10% protein, 0.7% carbohydrate); caloric density= 6.53 kcal/g
 JJR_Low Protein
Low protein(15% fat, 10% protein, 75% carbohydrate); caloric density= 3.77 kcal/g

Experimental Factor: Tissue Source

 serum
serum
Experimental Factor Values


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