MMPC :: Experiment

Experiment

Circulating insulin levels in B6 mice fed high fat diet with different animal
fat composition

Summary

Data Summary

Investigator	Vahmani, Payam
Description	Male C57BL/6J mice at 8 wk old were purchased from Jackson Laboratories and housed in a conventional animal room at Cole B facility under a controlled environment (23°C and 12-h light/dark cycle) with free access to diet and water. After 1 week of acclimation, the mice were divided into nine cages of similar average body weight (four mice per cage). Each cage were be randomly assigned for 12 weeks to either a control low fat diet D12450H; 10 kcal% Fat, Research Diets), a control high fat diet with lard (45 kcal% Fat; D12451 with lard ), or a high fat diet containing tallow (45 kcal% Fat; D12451 with tallow). Diets were prepared by Research Diets and were stored at -20 °C until use. Experimental protocols have been approved by the University of California, Davis Animal Use and Care Administrative Advisory Committee. The main objective of this study is to investigate the efffecs of different animal fats (pork vs beef) on obesity, insulin sensitivity, glucose tolerance, inflammation and gut microbiome in mice fed high-fat diets.
Status	Completed
Public Release	6/21/2023
Animal Age	Measured In: week(s) post-natal (w)
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Data Analysis

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Animals

Strain Name	Common Name	Females	Males	Unknown
C57BL/6J	C57BL/6J	0	36	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Feeding Group
	Fasted	Animals are deprived of food but not water.
	Fed	Animals are allowed to have food.
	Experimental Factor: Mouse Diet
	Research Diet- D12450H	Rodent Diet with 10% fat, 70% carbohydrate, 20% protein
	Research Diets - D12451	Research Diets - D12451 Rodent Diet with 45% kcal% fat
	Research Diets - D12451 (tallow)	Research Diets - D12451 Rodent Diet with 45% kcal% fat from tallow instead of lard
	Experimental Factor: Tissue Source
	plasma (EDTA)	plasma (EDTA)

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
insulin, plasma	insulin, plasma

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Mammalian Phenotype (MP) Terms

MPTermID	MPTermName	Definition	Other Results
MP:0002079	increased circulating insulin level	greater than normal levels of insulin in blood [ISBN:0-683-40008-8]	MMPC Search Results

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