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Experiment

2021-09-20 Olson - Ian 9 Mice 3 Days Run
Summary Data Summary
Investigator Olson, David
Description We are using Crispr technology to delete ALK from the PVH to assess its
influence on energy balance.
Status Completed
Public Release 12/16/2023
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagExperiment / ControlSame StrainNo dataNeed ConditionsNomenclature
Data Analysis
TypeCount
Animals9
Experimental Conditions4
Catalog Items1
Curation Info (# flags)5
Phenotype Assays2
Phenotype Measurements3834
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh/J
2
1
0
B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh Tg(Sim1-cre)1Lowl/J
5
1
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...

Experimental Factor: Virus Injected

 AAV8-Syn-DIO-mCherry
AAV8-hSyn-DIO-mCherry is a pre-made rAAV in serotype 8 which will Cre dependently over-express mCherry driven under human synapsin promoter.
 rAAV2-CMV-Alk_sgRNA
Alk-AAV serotype 2 virus
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

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