mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Strain
B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh Tg(Sim1-cre)1Lowl/J

Summary Data Summary
Official Name B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh Tg(Sim1-cre)1Lowl/J
Common Name B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh Tg(Sim1-cre)1Lowl/J
Description Sim1cre was crossed with Cas9GFP mice.
These Rosa26-LSL-Cas9 knockin mice have a floxed-STOP
cassette preventing expression of the downstream bicistronic
sequences (Cas9 and EGFP). Although under control of a CAG
promoter, widespread expression of cas9 and EGFP is
prevented by the STOP cassette. After exposure to Cre
recombinase, expression of cas9 and EGFP is observed.
Hemizygous mice are viable, fertile, normal in size, and do
not display any gross physical or behavioral abnormalities.
Transgene expression is observed in all areas that
endogenously express Sim1, including paraventricular
hypothalamus and other parts of the brain. When these
Sim1-Cre mice are bred with mice containing a loxP-flanked
sequence of interest, Cre-mediated recombination will result
in deletion of the flanked sequences in Sim1-expressing
tissues (including hypothalamus). As such, Sim1-Cre
transgenic mice may be useful in studying body weight
homeostasis, obesity, leptin metabolism, or as a reporter
strain for Sim1-transcription factor activity.
The Rosa26-LSL-Cas9 targeting vector used was designed with
(from 5' to 3') a 5' homology arm, a ubiquitously expressed
CAG promoter, loxP-flanked 3xSV40 polyA stop cassette, a
3X-FLAG epitope tag, a mammalian codon-optimized cas9 gene
(derived from Streptococcus pyogenes CRISPR associated
protein 9 [SpCas9]) flanked by two nuclear localization
signals, a P2A ribosomal skip cleavage peptide sequence, an
enhanced Green Fluorescent Protein (EGFP) gene, a woodchuck
hepatitis virus post-transcriptional regulatory element,
bovine growth hormone polyA, pPGK-Neo-pA positive selection
cassette and a 3' homology arm. The construct was
electroporated into 129S-derived R1 embryonic stem (ES)
cells. Correctly targeted ES cells were injected into
C57BL/6N blastocysts. The resulting chimeric animals were
tested for germline transmission by crossing to C57BL/6N
mice. The mice were then backcrossed for four generations
onto C57BL/6N.
Development Status Phenotyping ongoing
Creation Method transgenic
TypeCount
Investigators 1
Genomics - Transgenes 1
Experiments 3


Investigators
NameInstitution
David OlsonUniversity of Michigan-Ann Arbor


Genomic Information
EnhancerPromoterGeneCopies
Not SpecifiedCRESim1Not Specified


Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.