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Experiment

2020-11-08 Myers - Abigail 5 Days CLAMS Run w/ CNO Injections NMR
Summary Data Summary
Investigator Tomlinson, Abigail
Description Body composition before and after experiment. Mice were measured for body
composition before being put into CLAMS metabolic chambers on 11/8/20 at 3pm. On
11/9 and 11/10, all mice were injected with saline at 6am and 5pm. On 11/11 and
11/12, all mice were injected with clozapine-N-oxide at 6am and 5pm. On 11/13,
all mice received a saline injection at 6am before being taken out of the
metabolic chambers on the same day at 3:30pm and receiving the second body
composition.
Status Completed
Public Release 1/25/2024
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagExperiment / ControlNo dataNeed ConditionsNomenclature
Data Analysis
TypeCount
Animals12
Experimental Conditions4
Catalog Items2
Curation Info (# flags)4
Phenotype Assays7
Phenotype Measurements168
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
STOCK Lepr-flptm1.1KniGlp1rtm1.1(cre)Lbrl-Gt(ROSA)26Sortm3.2(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen/J
0
5
0
STOCK Lepr-flptm1.1KniGlp1rtm1.1(cre)Lbrl-Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze/J
3
4
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Clozapine N-oxide
Inactive from of clozapine drug, activates G-protein-coupled receptors (GPCRs). CNO serves as a synthetic ligand for engineered human muscarinic receptor. It elicits clozapine-like effects upon metabolic conversion. Clozapine conversion to CNO favors its

Experimental Factor: Experimental Group

 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...
Experimental Factor Values


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