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Experiment

2018-02-25 Olson - Ian 12 Mice
Summary Data Summary
Investigator Olson, David
Description Lab is investigating IRS4 expressing cells of the PVH and their potential role
in homeostasis. This could be translated to studying human obesity.

Mice were sent to the core for body composition (to measure body weight, fat,
lean, and fluid mass). Mice were then put into CLAMS machines for indirect
calorimetry measurements for several days. Respiration, activity, food, and
metabolic rates for each mouse were measured and compiled for lab's continued
research.
Status Completed
Public Release 6/17/2024
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals12
Experimental Conditions4
Catalog Items1
Curation Info (# flags)1
Phenotype Assays6
Phenotype Measurements144
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
3
0
IRS-4 KO
0
9
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet PicoLab Rodent Diet 5LOD
Crude Protein 23%, crude fat 4.5%, crude fiber 6.0%. Ingredients: dehulled soybean meal, ground corn, dried beet pulp, fish meal, ground oats, dehydrated alfalfa meal, cane molasses, brewers dried yeast, wheat germ, whey, porcine animal fat...

Experimental Factor: Virus Injected

 AAV-Flex-hM3Dq
A modified human muscarinic receptor coupled to the stimulatory Gq-protein which activates neurons upon binding the synthetic ligand clozapine N-oxide. Cre-dependent AAV-Flex-hM3Dq-mCherry (AAV-Flex-hM3Dq, titer: 4 × 1012 virus molecules/mL, purchased fro
 AAV-Flex-TetTox
Tetanus toxin cleaves synaptobrevin, a component of the SNARE core and prevents release of synaptic vesicles from targeted neurons.
Experimental Factor Values


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