mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Experiment

2022-08-22 Rui - Qianqian 8 Mice 3 Day CLAMS Run
Summary Data Summary
Investigator Rui, Liangyou
Description White adipose tissue (WAT) releases, via lipolysis, free fatty acids (FFA) that
serve as essential metabolic fuel to support life. Adipose triglyceride lipase
(ATGL) catalyzes the first rate-limiting step of lipolysis reactions.
Importantly, adipose ATGL expression and adipose lipolysis rates decrease during
aging and obesity, contributing to WAT expansion and metabolic dysfunctions.
However, the underlying mechanism for age/obesity-associated suppression of WAT
lipolysis remains poorly understood. Herein, we found that obesity is associated
with the upregulation of Mettl14, the essential component of RNA
N6-methyladenosine (m6A) methyltransferase complex in WAT.
Consequently, adipose m6A levels were higher in obese mice. Adipocyte-specific
deletion of Mettl14 markedly suppressed WAT growth and protected against high
fat diet-induced obesity, insulin resistance, and glucose intolerance. In
isolated WAT explants, lipolysis rates were significantly higher in
Mettl14-deficient than in wild-type adipocytes under both baseline and ß
adrenergic-stimulated conditions. Increased adipose lipolysis restrained WAT
expansion in adipocyte-specific Mettl14 knockout mice. Consistently, ATGL
protein levels were substantially higher in Mettl14-deficient than in wild-type
WAT. Remarkably, Mettl14 directly promoted ATGL mRNA m6A methylation in cell
cultures. Our results reveal a novel mRNA m6A methylation-based mechanism that
governs adipose lipolysis, WAT expansion, and obesity progression.
Status Completed
Public Release 8/25/2024
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals8
Experimental Conditions4
Catalog Items1
Curation Info (# flags)1
Phenotype Assays2
Phenotype Measurements3392
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
Mettl14
0
8
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 Research Diets - D12492
Rodent Diet with 34% Fat, 60% kcal from Fat

Experimental Factor: Strain Type

 Heterozygous
Having two different alleles of a particular gene or genes
 Homozygous
Having two identical alleles of a particular gene or genes.
Experimental Factor Values


Phenotype Assays Add / Edit


No documents found.

Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.