mmpc-logo
twitter-logo @NationalMMPC
| Create Account | login
Experiment

Hyperinsulinemic Clamp Order #2436 1/23/12
Summary Data Summary
Investigator Tobacman, Joanne
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups: WT

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.

We have demonstrated that addition of carrageenan to the water supply of the
C57BL/6J mice leads to glucose intolerance, insulin resistance, and impaired
insulin signaling.
Status Completed
Public Release 8/1/2014
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals16
Experimental Conditions4
Catalog Items8
Curation Info (# flags)2
Phenotype Assays19
Phenotype Measurements4661
Histology Images0
Publications0
Phenotypes1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
16
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Drug Administered

 carrageenan
carrageenan was added to the drinking water
 No Drug/Treatment
No Drug or Treatment done on these samples

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.



No documents found.

Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.