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Experiment

Hyperinsulinemic Clamp Order #2436 1/23/12
Summary Data Summary
Investigator Tobacman, Joanne
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups: WT

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.

We have demonstrated that addition of carrageenan to the water supply of the
C57BL/6J mice leads to glucose intolerance, insulin resistance, and impaired
insulin signaling.
Status Completed
Public Release 8/1/2014
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals16
Experimental Conditions4
Catalog Items8
Curation Info (# flags)2
Phenotype Assays19
Phenotype Measurements4661
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
16
0


Experimental Conditions
NameUnits
Drug Administeredone of [1GHI, 2-Fucosyllactose, ...]
Experimental Groupone of [Control, Control-Het, ...]
Insulin DosemiliUnits per kilogram (mU/kg)
Time Sample Takenminute (min)



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