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Experiment

Hyperinsulinemic Clamp Order #2436 1/23/12
Summary Data Summary
Investigator Tobacman, Joanne
Description Hyperinsulinemic-euglycemic clamp: Insulin is continuously infused at a constant
rate during 155min (see data for infusion rate), and glucose is infused at a
variable rate to maintain euglycemia. 3H-glucose is infused to assess glucose
fluxes. At 120min, a bolus of 14C-2-deoxyglucose is given to assess
tissue-specific glucose uptake. Mice are sacrificed at 155min.

Mouse were fasted for 5h prior to experiment.
Water: regular tap water, ad libitum.

Groups: WT

Purpose of the experiment: This experiment will determine whether the groups of
mice have different insulin sensitivity, and will determine sites of insulin
resistance.

Applicable Research Areas: Metabolism, Insulin resistance.

We have demonstrated that addition of carrageenan to the water supply of the
C57BL/6J mice leads to glucose intolerance, insulin resistance, and impaired
insulin signaling.
Status Completed
Public Release 8/1/2014
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals16
Experimental Conditions4
Catalog Items8
Curation Info (# flags)2
Phenotype Assays19
Phenotype Measurements4661
Histology Images0
Publications0
Phenotypes1


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
16
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Carrageenan
Carrageenan was added to the drinking water. Carrageenan are a family of linear sulfated polysaccharides that are extracted from red edible seaweeds. They are widely used in the food industry, for their gelling, thickening, and stabilizing properties. Th
 No Drug/Treatment
No Drug or Treatment done on these samples

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Insulin Dose (mU/kg)

 Insulin Dose

Experimental Factor: Time Sample Taken (min)

 Time Sample Taken
Time in minutes since a sample was taken


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