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Experiment

Oxymax Part 1 Order #3629 10/17/12
Summary Data Summary
Investigator Wasserman, David
Description Energy expenditure measured by indirect calorimetry in the Oxymax by Columbus
Instruments. Activity and food intake are also continuously measured.
Measurements were taken for 5 consecutive days.
Running wheels were not present in the cage.
Mice were fed ad libitum.
Water: regular tap water, ad libitum.

Groups:
Male WT (C57BL/6J) mice.
Mice were given either Vehicle-HN, a 0.01 mg/kg dose of PE6PH20, a 0.1 mg/kg
dose of PE6PH20, or a 1 mg/kg dose of PE6PH20.
Mice were fed a 60% kcal High Fat Diet (F3282 BioServ).

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure and feeding behaviors.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure, feeding behavior.
Status Completed
Public Release 10/18/2014
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals31
Experimental Conditions4
Catalog Items1
Curation Info (# flags)2
Phenotype Assays10
Phenotype Measurements310
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
31
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Drug Administered

 PEGPH20
Human Recombinant Hyduronidase P.H. 20 Drug Dose is in mg/kg body weight
 Vehicle-HN
10 mM Histidine, 130 mM NaCL at P.H. 6.5

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 BioServ F3282
Rodent Hig Fat Diet. Protein 20.%, Fat 35.5%, Fiber 0.0%, Ash 3.2%, Moisture <5.0%, Carbohydrate 36.3%. Ingredients: Lard, Casein, Dextrose, Sucrose, DL-Methionine, Choline Chloride, Vitamin Mix and Mineral Mix



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