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Experiment

Gut microbiome analysis- effect of colonization with E.coli strain
overexpressing enzyme to overproduce anorexigenic lipids
Summary Data Summary
Investigator Davies, Sean
Description the investigators engineered a bacteria strain to express an enzyme to enhance
the production of anorexigenic lipids. Mice on a high fat diet were given these
engineered bacteria (or control) as direct fed microbials in the drinking water
for 8 weeks, and then switched to normal water for 4 more weeks. We analyzed the
fecal microbiota of the mice treated with vehicle (0.125% gelatin), engineered
E.coli with control vector (pEcN), or E.coli with vector expressing
N-acetyltransferase (pNAPE-EcN).

Applicable research area(s): Metabolism
Status Completed
Public Release 2/14/2015
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals39
Experimental Conditions3
Catalog Items2
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements78
Histology Images0
Publications1
External References2
Documents1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
39
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 pEcN
pEcN is EcN with empty plasmid without NAPE as a control.
 pNAE
E. coli Nissle 1917 expressing pNAE to increase production of N-acylethanolamide (NAE) family of lipids
 pNAPES
Cloned Arabidopsis thaliana NAPE synthase (pNAPES).
 Vehicle
Vehicle - Control

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Total Time on Diet (wk)

 total time on diet
The total amount of time, in weeks, the subject spent on the particular diet.


Phenotype Assays Add / Edit


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