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Experiment

Gut microbiome analysis- effect of colonization with E.coli strain
overexpressing enzyme to overproduce anorexigenic lipids
Summary Data Summary
Investigator Davies, Sean
Description the investigators engineered a bacteria strain to express an enzyme to enhance
the production of anorexigenic lipids. Mice on a high fat diet were given these
engineered bacteria (or control) as direct fed microbials in the drinking water
for 8 weeks, and then switched to normal water for 4 more weeks. We analyzed the
fecal microbiota of the mice treated with vehicle (0.125% gelatin), engineered
E.coli with control vector (pEcN), or E.coli with vector expressing
N-acetyltransferase (pNAPE-EcN).

Applicable research area(s): Metabolism
Status Completed
Public Release 2/14/2015
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flaghas-doc-data-flagSame StrainAnimal Count
Data Analysis
TypeCount
Animals39
Experimental Conditions3
Catalog Items2
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements78
Histology Images0
Publications1
External References2
Documents1


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
39
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 pEcN
pEcN is EcN with empty plasmid without NAPE as a control.
 pNAE
E. coli Nissle 1917 expressing pNAE to increase production of N-acylethanolamide (NAE) family of lipids
 pNAPES
Cloned Arabidopsis thaliana NAPE synthase (pNAPES).
 Vehicle
Vehicle - Control

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor Values


Phenotype Assays Add / Edit




PublicationAltmetricsSubmitted ByPubMed IDStatus
Incorporation of therapeutically modified bacteria into gut microbiota inhibits obesity.
Chen Z, Guo L, Zhang Y, L Walzem R, Pendergast JS, Printz RL, Morris LC, Matafonova E, Stien X, Kang L, Coulon D, McGuinness OP, Niswender KD, Davies SS
The Journal of clinical investigation, 2014 (124), 3391 - 3406
24960158
Published


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