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Experiment

Energy expenditure in D36 mice
Summary Data Summary
Investigator Hell, Johannes
Description AKAP5 (also referred to as AKAP150 in rodents and AKAP79 in humans) is a
scaffolding protein that is highly expressed in neurons and targets a variety of
signaling molecules to dendritic membranes. AKAP5 interacts with PKA holoenzymes
containing RIIa or RIIß as well as calcineurin (PP2B), PKC, calmodulin, adenylyl
cyclase type V/VI, L-type calcium channels, and ß-adrenergic receptors. AKAP5
has also been shown to interact with members of the MAGUK family of
PSD-scaffolding proteins including PSD95 and SAP97 and target signaling
molecules to receptors and ion channels in the postsynaptic density (PSD). We
created two lines of AKAP5 mutant mice: a knockout of AKAP5 (KO) and a mutant
that lacks the PKA binding domain of AKAP5 (D36). We find that PKA is
delocalized in both the hippocampus and striatum of KO and D36 mice indicating
that other neural AKAPs cannot compensate for the loss of PKA binding to AKAP5.
In AKAP5 mutant mice, a significant fraction of PKA becomes localized to
dendritic shafts and this correlates with increased binding to microtubule
associated protein-2 (MAP2). Electrophysiological and behavioral analysis
demonstrated more severe deficits in both synaptic plasticity and operant
learning in the D36 mice compared with the complete KO animals. Our results
indicate that the targeting of calcineurin or other binding partners of AKAP5 in
the absence of the balancing kinase, PKA, leads to a disruption of synaptic
plasticity and results in learning and memory defects.
Status Completed
Public Release 2/14/2015
Animal Age Measured In: month(s) post-natal (m)
Flags has-data-flagExperiment Description
Data Analysis
TypeCount
Animals16
Experimental Conditions2
Catalog Items1
Curation Info (# flags)1
Phenotype Assays30
Phenotype Measurements480
Histology Images0
Publications0
Documents1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6
5
3
0
D36
6
2
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet 5058
PicoLab Mouse Diet 20, 20.0% protein (Calories: 23% protein, 22% Fat, 55% Carbohydrate)


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