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Experiment

Tissue Analysis # 6705
Summary Data Summary
Investigator Fretz, Jackie
Description White adipose tissue (WAT) morphology characterized by hypertrophy (i.e., fewer
but larger adipocytes) associates with increased adipose inflammation,
lipolysis, insulin resistance, and risk of diabetes. However, the causal
relationships and the mechanisms controlling WAT morphology are unclear. Herein,
we identified EBF1 as an adipocyte-expressed transcription factor with decreased
expression/activity in WAT hypertrophy. In human adipocytes, the regulatory
targets of EBF1 were enriched for genes controlling lipolysis and adipocyte
morphology/differentiation, and in both humans and murine models, reduced EBF1
levels associated with increased lipolysis and adipose hypertrophy. Although
EBF1 did not affect adipose inflammation, TNFa reduced EBF1 gene expression.
High-fat diet intervention in Ebf1(+/-) mice resulted in more pronounced WAT
hypertrophy and attenuated insulin sensitivity compared with wild-type
littermate controls. We conclude that EBF1 is an important regulator of adipose
morphology and fat cell lipolysis and may constitute a link between WAT
inflammation, altered lipid metabolism, adipose hypertrophy, and insulin
resistance.

Applicable research area(s): Diabetes, Endocrine deficiency, Metabolism
Status Completed
Public Release 1/31/2015
Data Collected? Data will not be collected for this catalog item
Animal Age Measured In: month(s) post-natal (m)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals11
Experimental Conditions1
Catalog Items3
Curation Info (# flags)2
Phenotype Assays3
Phenotype Measurements33
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6
0
0
2
B6;129-Ebf1tm1Rug
0
0
9

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.
Experimental Factor (Units)
Experimental Group
Experimental Factor Values


No documents found.

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