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Strain
B6;129-Ebf1tm1Rug

Summary Data Summary
Official Name B6;129-Ebf1tm1Rug
Common Name B6;129-Ebf1tm1Rug
Description Murine genomic EBF clones were isolated from a 129/Sv strain
genomic library. An 11.2 kb genomic DNA containing 5' exons
was subcloned into pBluescript to generate the gene
targeting construct in which a 3.4 kb BamHI fragment was
replaced with the 1.4 kb PGK::neo gene cassette, which was
subsequently inserted into a blunt-ended SalI site. Clones
were doubly elected with neomycin and FIAU for the presence
of the neo gene and loss of the TK gene. Chimeric mice were
generated and homozygotes were used for the experiment.
Development Status Phenotyping ongoing
Creation Method knockout
Breeding Type intercross
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 2


Investigators
NameInstitution
Jackie FretzYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Ebf1knockoutknockoutNot Specified


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