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Experiment

Metabolic Clamp Study in JNK2 KO Mice

Summary

Data Summary

Investigator	Davis, Roger J.
Description	The JNK signaling pathway is implicated in the regulation of the AP1 transcription factor and cell proliferation. Here, we examine the role of JNK by using conditional and chemical genetic alleles of the ubiquitously expressed murine genes that encode the isoforms JNK1 and JNK2. Our analysis demonstrates that JNK is not essential for proliferation. However, JNK is required for expression of the cJun and JunD components of the AP1 transcription factor, and JNK-deficient cells exhibit early p53-dependent senescence. These data demonstrate that JNK can act as a negative regulator of the p53 tumor suppressor. Applicable research area(s): Diabetes, Obesity, Metabolism
Status	PhenStat Analysis Verified
Public Release	9/25/2015
Animal Age	Measured In: month(s) post-natal (m)
Flags

Data Analysis

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Type	Count
Animals	30
Experimental Conditions	2
Catalog Items	4
Curation Info (# flags)	3
Phenotype Assays	14
Phenotype Measurements	480
Histology Images	0
Publications	0
Phenotypes	9

Animals

Strain Name	Common Name	Females	Males	Unknown
B6.129-Mapk9^tm1.1Rjd/J	B6.129-Jnk2^tm1.1Rjd/J	0	30	0

Animals/Strains

Experimental Factors

	Categorical Values
	Name / Abbreviation	Description
	Experimental Factor: Experimental Group
	Control	This animal belongs to the control group for the experiment.
	Experiment	This animal belongs to experimental group that is homozygous for gene manipulations.
	Experimental Factor: Mouse Diet
	LabDiet 5P75/5P76	Prolab 5P75 and 5P76 Isopro 3000 are the irradiated forms of RMH 3000 (5P00). The product has been irradiated to virtually bacteria-free dietary control. Protien 22.5%, Fat 5.4% Cholesterol 195 ppm
	Teklad - 93075	Harlan-Teklad TD.93075 Adjusted Calorie diet (Calories: 21% protein, 55% Fat, 24% Carbohydrate) A diet used to induce obesity. Approximate fatty acid profile (% total fat): 28% saturated, 30% trans, 28% monounsaturated (cis), 14% polyunsaturated...

Experimental Factor (Units)
Experimental Group
Mouse Diet

Experimental Factor Values

Phenotype Assays Add / Edit

Name	Abbreviation
body mass	W rodent
fat body mass	fat body mass
lean body mass	lean body mass
glucose infusion rates	glucose infusion rates
glucose, basal	glucose, basal
hepatic glucose production, basal	hepatic glucose production, basal
glucose turnover, whole body	glucose turnover, whole body
glycogen/lipid synthesis, whole body	glycogen/lipid synthesis, whole body
glycolysis, whole body	glycolysis, whole body
glucose, clamp average	glucose, clamp average
Hepatic Glucose Production, clamp	Hepatic Glucose Production, clamp
glucose uptake, muscle	glucose uptake muscl
glucose uptake, white adipose	glucose uptake white
glucose uptake, brown fat	glucose uptake brown

No documents found.

Mammalian Phenotype (MP) Terms

MPTermID	MPTermName	Definition	Other Results
MP:0001260	increased body weight	greater than normal average weight [J:33400]	MMPC Search Results
MP:0003960	increased lean body mass	greater amount of the fat-free physical bulk or volume of the body including all its components except adipose (fat) tissue [MGI:csmith]	MMPC Search Results
MP:0005560	decreased circulating glucose level	less than the normal concentration in the blood of this major monosaccharide of the body; it is an important energy source [MGI:cwg\| RGD:cur]	MMPC Search Results
MP:0010024	increased total body fat amount	greater than the normal total amount of connective tissue composed of fat cells within the entire body [MGI:csmith]	MMPC Search Results
MP:0014142	increased body fat mass	increased physical bulk or volume of fat in the whole body [MGI:csmith]	MMPC Search Results
MP:0020102	increased hepatic glucose production	increased production of glucose in the liver [https://orcid.org/0000-0001-5208-3432]	MMPC Search Results
MP:0020103	decreased hepatic glucose production	decreased production of glucose in the liver [https://orcid.org/0000-0001-5208-3432]	MMPC Search Results
MP:0030017	decreased adipocyte glucose uptake	reduced ability of adipocytes to take in glucose [MGI:anna]	MMPC Search Results
MP:0030022	decreased muscle cell glucose uptake	reduced ability of muscle cells to take in glucose [MGI:anna]	MMPC Search Results

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