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Experiment

Metabolic Clamp Study in JNK2 KO Mice
Summary Data Summary
Investigator Davis, Roger J.
Description The JNK signaling pathway is implicated in the regulation of the AP1
transcription factor and cell proliferation. Here, we examine the role of JNK by
using conditional and chemical genetic alleles of the ubiquitously expressed
murine genes that encode the isoforms JNK1 and JNK2. Our analysis demonstrates
that JNK is not essential for proliferation. However, JNK is required for
expression of the cJun and JunD components of the AP1 transcription factor, and
JNK-deficient cells exhibit early p53-dependent senescence. These data
demonstrate that JNK can act as a negative regulator of the p53 tumor
suppressor.

Applicable research area(s): Diabetes, Obesity, Metabolism
Status Completed
Public Release 9/25/2015
Animal Age Measured In: month(s) post-natal (m)
Flags has-data-flagSame StrainAnimal CountExperiment Description
Data Analysis
TypeCount
Animals30
Experimental Conditions2
Catalog Items4
Curation Info (# flags)3
Phenotype Assays14
Phenotype Measurements480
Histology Images0
Publications0
Phenotypes9


Animals

Strain NameCommon NameFemalesMalesUnknown
B6.129-Jnk2tm1.1Rjd/J
0
30
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet 5P75/5P76
Prolab 5P75 and 5P76 Isopro 3000 are the irradiated forms of RMH 3000 (5P00). The product has been irradiated to virtually bacteria-free dietary control. Protien 22.5%, Fat 5.4% Cholesterol 195 ppm
 Teklad - 93075
Harlan-Teklad TD.93075 Adjusted Calorie diet (Calories: 21% protein, 55% Fat, 24% Carbohydrate) A diet used to induce obesity. Approximate fatty acid profile (% total fat): 28% saturated, 30% trans, 28% monounsaturated (cis), 14% polyunsaturated...



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