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New comment to be added:
Gut microbiome analysis in wild-type C57BL/6J chow-fed mice: Effect of gut
location(biogeography) and sequencing method on microbiota diversity
To characterize the bacterial community based on gut location in chow-fed mice
and to determine if different sequencing platforms (454, Illumina, and Ion PGM)
and different primers give comparable results with the same samples.
10 C57BL/6 male mice- Individually housed with Care-Fresh bedding Diet: Harlan
Teklad Global 18% protein rodent diet #2918 (Chow). Samples collected and
tested: 1. Feces 2-24h (48h) cage collected fecal samples (pooled); 2. Single
pellet of freshly collected feces; 3. Formed fecal pellet from colon; 4. Small
Intestine contents; 5. Cecum contents; 6. Large Intestine contents;
Platforms/Primers tested: 454-27F-519R; 454- 515F-806R;
Illumina-2x300bp-515F-806R; Ion PGM-515F-806R.
week(s) post-natal (w)
DOWNLOAD ALL DATA (.csv)
Curation Info (# flags)
Name / Abbreviation
Experimental Factor: Tissue Source
feces - cage
Feces collected from cage
feces - colon
feces collected from colon
feces - fresh
feces - fresh
Experimental Factor (Units)
Add / Edit
Add / Edit
Link to Reference
Sequence Read Archive (SRA)
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European Nucleotide Archive
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
) under the MICROMouse Program, grants DK076169.
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