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Experiment

Gut microbiome analysis in wild-type C57BL/6J chow-fed mice: Effect of gut
location(biogeography) and sequencing method on microbiota diversity
Summary Data Summary
Investigator Knotts, Trina
Description To characterize the bacterial community based on gut location in chow-fed mice
and to determine if different sequencing platforms (454, Illumina, and Ion PGM)
and different primers give comparable results with the same samples.

10 C57BL/6 male mice- Individually housed with Care-Fresh bedding Diet: Harlan
Teklad Global 18% protein rodent diet #2918 (Chow). Samples collected and
tested: 1. Feces 2-24h (48h) cage collected fecal samples (pooled); 2. Single
pellet of freshly collected feces; 3. Formed fecal pellet from colon; 4. Small
Intestine contents; 5. Cecum contents; 6. Large Intestine contents;
Platforms/Primers tested: 454-27F-519R; 454- 515F-806R;
Illumina-2x300bp-515F-806R; Ion PGM-515F-806R.
Status Completed
Public Release 1/22/2017
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagAnimal CountNo Experimental Group
Data Analysis
TypeCount
Animals10
Experimental Conditions1
Catalog Items3
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements60
Histology Images0
Publications0
External References2
Documents2


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J
0
10
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Tissue Source

 caecum
caecum
 feces - cage
Feces collected from cage
 feces - colon
feces collected from colon
 feces - fresh
feces - fresh
 large intestine
large intestine
 small intestine
small intestine
Experimental Factor (Units)
Tissue Source


Phenotype Assays Add / Edit



No documents found.

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