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Experiment

Measurement of energy expenditure at different temperatures in WT and RyR1-R163C
heterozygotes (thermoneutral zone testing)
Summary Data Summary
Investigator Pessah, Isaac
Description Examine effect of Ryr1 mutation on energy expenditure in temperature-challenged
female mice.


MH is a life-threatening pharmacogenetic syndrome, which occurs when susceptible
individuals are exposed to triggering agents, such as halogenated inhalation
anesthetics and/or depolarizing muscle relaxants. MH susceptibility is
associated with a dysfunction of regulation of intracellular resting Ca2+
concentration ([Ca2+]r) and is characterized by the occurrence of a robust
increase in [Ca2+]r in response to exposure to halothane. In more than 70% of
humans with MH susceptibility (MHS) the trait is linked to one of >185 mutations
within RYR1 located on ch19q13.1, the gene that encodes the type 1 skeletal
isoform of the ryanodine receptor (RYR1), the primary Ca2+ release channel in
the sarcoplasmic reticulum (SR). In addition, mutations in CACNA1S on ch1q31–32,
which codes for CaV1.1 have also been found in 2% of MHS individuals.

Applicable research area(s): Cardiovascular, Metabolism
Status Completed
Public Release 4/14/2017
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals12
Experimental Conditions3
Catalog Items1
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements84
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+
6
0
0
RyR1-R163C
6
0
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Environmental Temperature (°C)

 env temp
The temperature of the environment when the measurement was taken.

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment-Het
This animal belongs to the experimental group and is heterozygous wild type/mutation for the affected locus.

Experimental Factor: Mouse Diet

 Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...


Phenotype Assays Add / Edit



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