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Strain
B6.Cg-Ryr1tm2.1Alle/+

Summary Data Summary
Official Name B6.Cg-Ryr1tm2.1Alle/+
Common Name RyR1-R163C
Description Four nucleotides in exon 6 were modified (CgAgttggGgaT to
TgCgttggAgaC) to encode an Arginine to Cysteine change of
codon 163 as well as to generate a new BsmBI restriction
site. In addition, a floxed neomycin cytosine deaminase
cassette was inserted in the EagI site in intron 6. Germline
deletion of the selection cassette was subsequently
accomplished in the germ line by breeding to
Alp1tm1(cre)Nagy mice.
Development Status Phenotyping ongoing
Creation Method knockin
Breeding Type backcross
TypeCount
Investigators 1
Genomics - Modifications 1
Experiments 12


Investigators
NameInstitution
Isaac PessahUniversity of California - Davis


Genomic Information
GeneAllele 1Allele 2Protocol
Ryr1knockinwildNot Specified


OrderInvestigatorExperiment StatusMeasurements
2073620736Isaac Pessah
Analysis of energy expenditure and core temperature during temperature challenge in a model of malignant hyperthermia
Completed6160
2213822138Isaac Pessah
Assessment of RyR1 mutant mouse activity
Completed2925
2213822138Isaac Pessah
Effect of Ryr on running bouts
Completed450
2560925609Isaac Pessah
Effects of Ryanadine receptor hemizygosity on energy expenditure with running wheel
Completed22200
1849418494Isaac Pessah
Energy expenditure in WT and RyR1 R163C mice during acute heat stress
Completed732
1849318493Isaac Pessah
Examine effect of Ryr1 mutation on energy expenditure in temperature-challenged male and female mice (2nd cohorts)
Completed296
1849218492Isaac Pessah
Examine effect of Ryr1 mutation on energy expenditure in temperature-challenged male mice (male cohort1)
Completed92
1317413174Isaac Pessah
Measurement of energy expenditure at different temperatures in WT and RyR1-R163C heterozygotes (thermoneutral zone testing)
Completed84
1317413174Isaac Pessah
Measurement of standard energy expenditure at 22C in Control and RyR1-R163C Heterozygotes
Completed336
1849318493Isaac Pessah
Measurement of standard energy expenditure at 22C in Control and RyR1-R163C Heterozygotes (female cohorts 2 &3, male cohort2)
Completed744
1849218492Isaac Pessah
Measurement of standard energy expenditure at 22C in Control and RyR1-R163C Heterozygotes (MALES-cohort1)
Completed248
1332213322Jennifer Rutkowsky
Temperature by telemetry during CLAMS analysis
Completed64812

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