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Experiment

Effects of Ryanadine receptor hemizygosity on energy expenditure with running
wheel
Summary Data Summary
Investigator Pessah, Isaac
Description Mutations in Either type 1 receptor (RyR1) or the dihydropyridine
receptorsubunit Cav1.1 cause malignant hyperthermia susceptiblity (MHS) in
humans and animals. It is necessary to understand how altered RyR1 channel
regulation influences baal changes in mitochondrial bioenergetics, produces
oxidative stress and promotes progressive muscle change. This study is a
follow-up study to the running wheel study done in male and female mice (WT and
HET). Here, energy expenditure (by indirect calorimetry) and core temperature
readings are assessed while allowed access to voluntary running wheels at two
environmental temperatures (22 and 28 C). This will allow us to better
characterize how ambient temperature influences heat stress in MHS mice females
when compared to males.

Applicable research area(s): Metabolism
Status Completed
Public Release 1/9/2021
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flag
Data Analysis
TypeCount
Animals30
Experimental Conditions4
Catalog Items3
Curation Info (# flags)0
Phenotype Assays37
Phenotype Measurements22200
Histology Images0
Publications0


Animals
Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+
8
7
0
RyR1-R163C
8
7
0

Animals/Strains
Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment-Het
This animal belongs to the experimental group and is heterozygous wild type/mutation for the affected locus.

Experimental Factor: Light-Dark Period

 Dark
The Dark period
 Light
The light period
Experimental Factor Values


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