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Experiment

Energy expenditure in WT and RyR1 R163C mice during acute heat stress
Summary Data Summary
Investigator Pessah, Isaac
Description Whole body calorimetry will be used to measure energy expenditure and how this
is altered by acute heat stress. Blood and tissues will be dissected to explore
molecular changes involved in heat stress.

MH is a life-threatening pharmacogenetic syndrome, which occurs when susceptible
individuals are exposed to triggering agents, such as halogenated inhalation
anesthetics and/or depolarizing muscle relaxants. MH susceptibility is
associated with a dysfunction of regulation of intracellular resting Ca2+
concentration ([Ca2+]r) and is characterized by the occurrence of a robust
increase in [Ca2+]r in response to exposure to halothane. In more than 70% of
humans with MH susceptibility (MHS) the trait is linked to one of >185 mutations
within RYR1 located on ch19q13.1, the gene that encodes the type 1 skeletal
isoform of the ryanodine receptor (RYR1), the primary Ca2+ release channel in
the sarcoplasmic reticulum (SR). In addition, mutations in CACNA1S on ch1q31–32,
which codes for CaV1.1 have also been found in 2% of MHS individuals.

Applicable research area(s): Cardiovascular, Metabolism
Status Completed
Public Release 4/15/2017
Animal Age Measured In: day(s) post-natal (d)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals36
Experimental Conditions3
Catalog Items1
Curation Info (# flags)1
Phenotype Assays7
Phenotype Measurements732
Histology Images0
Publications0
Documents3
Phenotypes1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+
0
19
0
RyR1-R163C
0
17
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment-Het
This animal belongs to the experimental group and is heterozygous wild type/mutation for the affected locus.

Experimental Factor: Mouse Diet

 Teklad - 2918
Harlan-Teklad 2918 (Irradiated) 18.6% protein (Calories : 24% protein, 18% Fat, 58% Carbohydrate) Ingredients—Ground wheat, ground corn, wheat middlings, dehulled soybean meal, corn gluten meal, soybean oil, calcium carbonate, brewers dried yeast,...



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