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Publication
Vertical sleeve gastrectomy reduces blood pressure and hypothalamic endoplasmic
reticulum stress in mice.
Authors McGavigan AK, Henseler ZM, Garibay D, Butler SD, Jayasinghe S, Ley RE, Davisson
RL, Cummings BP
Submitted By Submitted Externally on 4/17/2017
Status Published
Journal Disease models & mechanisms
Year 2017
Date Published
Volume : Pages 10 : 235 - 243
PubMed Reference 28093508
Abstract Bariatric surgery, such as vertical sleeve gastrectomy (VSG), causes remarkable
improvements in cardiometabolic health, including hypertension remission.
However, the mechanisms responsible remain undefined and poorly studied.
Therefore, we developed and validated the first murine model of VSG that
recapitulates the blood pressure-lowering effect of VSG using gold-standard
radiotelemetry technology. We used this model to investigate several potential
mechanisms, including body mass, brain endoplasmic reticulum (ER) stress
signaling and brain inflammatory signaling, which are all critical contributors
to the pathogenesis of obesity-associated hypertension. Mice fed on a high-fat
diet underwent sham or VSG surgery and radiotelemeter implantation. Sham mice
were fed ad libitum or were food restricted to match their body mass to
VSG-operated mice to determine the role of body mass in the ability of VSG to
lower blood pressure. Blood pressure was then measured in freely moving
unstressed mice by radiotelemetry. VSG decreased energy intake, body mass and
fat mass. Mean arterial blood pressure (MAP) was reduced in VSG-operated mice
compared with both sham-operated groups. VSG-induced reductions in MAP were
accompanied by a body mass-independent decrease in hypothalamic ER stress,
hypothalamic inflammation and sympathetic nervous system tone. Assessment of gut
microbial populations revealed VSG-induced increases in the relative abundance
of Gammaproteobacteria and Enterococcus, and decreases in Adlercreutzia These
results suggest that VSG reduces blood pressure, but this is only partly due to
the reduction in body weight. VSG-induced reductions in blood pressure may be
driven by a decrease in hypothalamic ER stress and inflammatory signaling, and
shifts in gut microbial populations.




Strains
StrainDevelopment StatusCreation MethodOptions
C57BL/6JNot ApplicableNot specified
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