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Publication
Prebiotic milk oligosaccharides prevent development of obese phenotype,
impairment of gut permeability and microbial dysbiosis in high-fat fed mice.
Authors Hamilton MK, Ronveaux CC, Rust BM, Newman JW, Hawley M, Barile D, Mills DA,
Raybould HE
Submitted By Submitted Externally on 5/1/2017
Status Published
Journal American journal of physiology. Gastrointestinal and liver physiology
Year 2017
Date Published
Volume : Pages Not Specified : ajpgi.0042
PubMed Reference 28280143
Abstract Microbial dysbiosis and increased intestinal permeability is a target for
prevention or reversal of weight gain in high-fat (HF) diet-induced obesity
(DIO). Prebiotic milk oligosaccharides (MO) have been shown to benefit the host
intestine, but have not been used in DIO. We hypothesized that supplementation
with bovine MO would prevent the deleterious effect of HF diet on the gut
microbiota and intestinal permeability, and attenuate development of the obese
phenotype. C57BL/6 mice were fed a control diet (LF), HF (40% fat/kcal), or HF +
prebiotic (6%/Kg BMO or inulin) for 1, 3 or 6 weeks. Gut microbiota and
intestinal permeability were assessed in the ileum, cecum and colon. Addition of
BMO to the HF diet significantly attenuated weight gain, decreased adiposity and
decreased caloric intake; inulin supplementation also lowered weight gain and
adiposity, but this did not reach significance. BMO and inulin completely
abolished the HF diet-induced increase in paracellular and transcellular
permeability in the small and large intestine. Both BMO and inulin increased
abundance of beneficial microbes Bifidobacterium and Lactobacillus in the ileum.
However, inulin supplementation altered phylogenetic diversity and decreased
species richness. We conclude that addition of BMO to the HF diet completely
prevented increases in intestinal permeability and microbial dysbiosis and was
partially effective to prevent weight gain in DIO.




Strains
StrainDevelopment StatusCreation MethodOptions
C57BL/6JNot ApplicableNot specified
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